Mifepristone Inhibition Of Androgen-independent Prostate Cancer Pc-3 Cell Proliferation In Vitro Experimental Study

Objective To study the effect of mifepristone on the proliferation, cell cycle, apoptosis, morphological and structural changes in the prostate cancer cell line PC-3 in vitro. Methods Prostate cancer cell line PC-3 was cultured with mifepristone of different concentration (1μmol/L-100μmol/L) in vitro. MTT was a technique to examine the cell proliferation rate, and FCM was to determine the phase of cell cycle and apoptosis rate, electron microscope was applied to detect the morphological and structural changes of the prostate cancer cell.Results (1) The A values of the cancer cells treated with 1μmol/L of mifepristone weresimilar to that of controls (P>0.05), while with 5μmol/L-100μmol/L of mifepristone were significantly different from controls (P<0.01). Mifepristone markedly inhibited cell proliferation of prostate cancer cell PC-3 on a dose- and time- depending manner. (2) Flow cytometry analysis showed the prostate cancer cells treated with mifepristone were blocked in cell cycle progression at G₁/ G₀ phase and decreased at S & G₂/M phase. The early apoptosis rates of prostate cancer cells treated with mifepristone were increased in dose-dependent manner, which was a significantly different from that of controls (P<0.01). (3) Cell morphology changes: It had early apoptosis changes in PC-3 cells treated with 10μmol/L of mifepristone at 48h by light microscope. With increasing drug concentration and time, cell apoptosis morphology changes...