Biological Characteristics Of Cord Blood Source Of Adherent Cells And Mononuclear Cells In The Bone Marrow Cavity Transplantation In Nod / Scid Mice

The present study was designed to explore biological characteristics of adherent cells (ACs) derived from umbilical cord blood and to determine if intra-bone marrow injection (iBM) of human umbilical cord blood mononuclear cells reconstitute hematopoiesis in NOD/SCID mice.In PART â…  , the biological characteristics of adherent cells (ACs) derived from umbilical cord blood (UCB) and the mRNA expression of some cytokines promoting hematopoiesis on these ACs were investigated. The low-density mononuclear cells (MNC) from UCB of normal full term deliveries were isolated by Ficoll-Hypaque and cutured with low glucose DMEM including 10% fetal bovine serum. The morphology, proliferation curve, cell cycle, expressions of adhesive molecules, and differentiation of mesenchymal-like stem cells (MSC) and the mRNA expression of some cytokines were assessed. MSC coud be isolated and cutured from some (6/37) UCB samples. These cells displayed fibroblast-like morphology. Cell cycle analysis revealed that more than 90% of MSC cells were in G₀/G₁ phase. They withstood being passaged over 31 times with no significant structural changes found. Expression of adhesive molecules (ADMs) such as CD₁₃, CD₂₉, CD₄₄ and CD₁₀₅, but not CD₁₄, CD₃₁, CD₃₄ and CD₁₀₆, was observed. Exposure of these cells to agents for inducing differentiation resulted in a formation of osteoblasts and adipocytes.