Man Testicular Protein Expression Profile Build And Sperm Proteomics Research

Testis is the major male gonad with responsibility for producing androgen and spermatozoon. A complete understanding of proteins expressed in the human testis will provide biological information on mechanism underlying spermatogenesis and male reproductive regulation. The purposes of this study were to apply a proteomic approach to investigating protein composition and to establish a 2-D PAGE reference map for human testis proteins. When total protein was assayed by 2-D PAGE and stained with sliver, about 2000 protein spots with a pI of pH 3-10 and Mr of 12 - 120 kDa could be detected. All these protein spots were excised for identification, among which 979 spots corresponding to 541 different proteins were identified undoubtful by searching the mass spectra against the SwissProt/TrEMBL database. A 2-D PAGE-based human testis protein database has been constructed on the basis of these results and published on the Internet(http://reprod.njmu.edu.cn/cgi-bin/2d/2d.cgi). This database is a federated 2-D PAGE database, could be linked from WORLD-2DPAGE index. All the identified proteins were bioinformatically annotated in detail based on their molecular function, biological process etc. About 80% of these proteins play roles in generalized metabolism process. Testis characteristic metabolic pathways have been analysed according to Kegg and BioCarta pathway databases. The significant pathways both exist in the results give by these two databases including glycolysis pathway and arginine...