The Study Of Human Epidermal Growth Factor Receptor In Tumor Biological Significance Of The Functional Expression Of E. Coli Membrane And The P38 Kinase Signal Transduction Pathways

Functional Expression of the Human Epidermal Growth Factor Receptor in Escherichia Coli: a new Method for Analyzing the Biological Activity of EGF Isoforms. The members of EGF family ,which include epidermal growth factor (EGF), transforming growth factor- α (TGF-α ), amphiregulin(AR), hepann-bindmg EGF-like growth factor (HB-EGF), betacelluhn(BTC),and epiregulin, share the same receptor , the EGF-R , and a spectrum of biological activities. In order to investigate the physiologic and pathologic role of the EGF family in digestive diseases and cancers, we try to establish a system that could quantitatively analyze the integral biological function of the EGF family. The main strategy of the study is to obtain an E coli strain which expresses the extracellular domain III of the human EGF-R (EGF-R III), which mainly contributes to ligand-binding, in its membrane, by which we might create a method to measure the biological activity of EGF family according to the theory that multi-ligands bind competitively to the same receptor. It contained three steps: firstly, to clone the DNA of EGF-R III; secondly,to construct the expressing vector pLERUI in...