Xiaoyin Detoxification Drink The Treatment Of Psoriasis Vulgaris Cell Biology Research

Psoriasis(PS) is a chronic inflammatory dermatosis characterized by erythema and scales. As a common disease throughout the world it affects patients'physical and mental health greatly. The aetiology of PS is not clear yet and the curative effect is not very satisfatory. Usually, it has a protract course, liable to relapse. Modern researches have established hyperkeratinization and parakeratosis of keratinocytes(KC) and the hyperplasia of the capillary vessel in the dermis as the fundamental pathological changes.Xiao Yin Jie Du Decocction (XYJDY) is an effective herbal medicine manufactured by our hospital and it has been used to treat PS for more than twenty years in our department. Clinic studies finished by our department recently has shown that the total effective rate is 91.8%, more than that of the control group using Fu Fang Qing Dai Capsule. Interleukin-8(IL-8) in the scales of psoriasis patients decreased significantly after four weeks'treatment of XYJDY. Animal experiment indicated that XYJDY could also inhibit IL-8 production and antagonize IL-8 increase induced by SEB in mouse's blood. In order to study the effect of XYJDY on KC and the endothelium of hypoderma and observe the curative effect of three main components group of XYJDY (cooling blood Group,detoxicating Group,expelling wind and removing dampness Group), Using MTT chromatometry, we studied the effect of mouse serum (treated with XYJDY and modified decoction) on the multiplication of Malpighian cell COLO-16 and human navel intravenous endotheliocyte ECV-304. We studied their apoptosis with flow cytometry and VEGF secretion of malpighian cell with double antibody ABC-ELISA method. We probed into the mechanism and the target site of XYJDY in treating PS by using the cytobiological research methods in order to explore new methods for the clinic research of PS and theorectical research of syndrome differentiation.The findings suggested that: 1.XYJDY and cooling blood group could significantly restrain the proliferation of KC The higher concentration of decoction was , the lower the survival rate would be . The survival rate of KC in XYJDY group (concentration of 5% and 10% ) was significantly lower than that in the control group of distilled water (P<0.05), and even lower when the concentration of XYJDY is 20%(P<0.001). The survival rate of KC in cooling blood group was significantly lower than that in the control group when the concentration of the herb was 10%(P<0.05), and even lower when the concentration was 20% (P<0.001). The survival rate of KC in detoxicating group or expelling wind and removing dampness group was not statistically different from that in the control group, but the higher concentration of decoction was , the lower survival rate became. There was significant difference (P<0.001) in the survival rate of KC between MTX group and the control group when the concentration of MTX was higher than 10%. 2. The apoptosis of KC in detoxicating group, expelling wind and removing dampness group and cooling blood group was not significantly different from that in the control when the concentration was 20%. The apoptosis of KC in XYJDY group or MTX group was significantly higher than that in the control group (distilled water) (P<0.001) 3. With a serum medicine concentration of 5%, the sruvival rate of endothelial cell in MTX group,XYJDY group and cooling blood group were all lower than that in the control group, but sruvival rate of endothelial cell in MTX group only was significantly lower than that in the control group(P<0.05). When the concentration of medicine reached 10%, the sruvival rate of endothelial cell in MTX group and XYJDY group were extremely significantly lower than that in the control group(P<0.001), and in cooling blood Fang group it was significantly lower than that in the control group(P<0.05). When the concentration of medicine reached 20%, the sruvival rate of endothelial cell in MTX group,XYJDY group and cooling blood Fang group were all extremely significantly lower than that in the control group(P<0.001), and in expelling wind and removing dampness group it was significantly lower than that in the control group(P<0.05). 4. When the concentration of medicine was 5%, XYJDY and MTX group could restrain the VEGF released by colo-16 cell in vitro, and the level of VEGF in XYJDY group was significantly lower than that in the control group. When the concentration of medicine was 10%, XYJDY group,MTX group and detoxicating group could restrain the VEGF released by colo-16 cell in vitro, and the level of VEGF in XYJDY group and detoxicating group was significantly lower than that in the control group. When the concentration of medicine was 20%, XYJDY group,cooling blood group,MTX group and detoxicating group could all restrain the VEGF released by colo-16 cell in vitro, and the level of VEGF in XYJDY group,cooling blood group and detoxicating group was significantly lower than that in the control group. The findings shows that VEGF level has an inverse association with serum concentration of medicine in XYJDY group,cooling blood group,MTX group and detoxicating group.The result indicates that:1. XYJDY can restrain the hyperplasia of keratinocyte and induce apoptosis of keratinocyte in psoriasis patients. 2. XYJDY can also restrain the hyperplasia of vascular endothelial cell of psoriasis patients. 3. XYJDY can inhibit the production of VEGF, released by keratinocyte. 4. By separating XYJDY to three main component groups to observe the effect of them on psoriasis. We find that cooling blood group can restrain the hyperplasia of keratinocyte and blood vessel endothelium cell while detoxicating group can inhibit the production of VEGF released by keratinocyte, and that the combination of three groups could enhance these effects significantly. 5. The findings indicated that the curative effect of XYJDY on psoriasis is probably mediated by inhibiting KC proliferation,inducing apoptosis of keratinocyte and restraining the hyperplasia of dermis capillary vessel. We postulate that XYJDY's therapeutic effect on psoriasis is probably realized through different target sites.