The Effect And Mechanical Study Of Vascular Endothelial Growth Factor On The Apoptosis Of Vascular Endothelial Cell

Objective To study the mechanical of vascular endothelial growth factor (VEGF) on prevention of restenosis after percutaneous coronary intervention (PCI) by observing the effect of VEGF on the apoptosis and the expression of Bcl-2 mRNA and Fas mRNA of vascular endothelial cell induced by hypoxia and H₂O₂Method Human umbilical vein endothelial cells (hUVEC) were divided into control group, hypoxia-treated group, H₂O₂-treated group, hypoxia +VEGF-treated group and H₂O₂ +VEGF-treated group. Apoptosis of hUVEC was determined by FCM and TUNEL, the expression of Bcl-2 mRNA and Fas mRNA was examined by RT-PCR.Result In hypoxia-treated and H₂O₂-treated group, the apoptosis cell and the expression of Fas mRNA were significantly increased, the expression of Bcl-2 mRNA was markedly decreased, but in VEGF-treated group, the changes of apoptosis cell and gene expression were inverseConclusion VEGF could inhibit the apoptosis of hUVEC induced by hypoxia and H₂O₂ via downregulation of Fas mRNA expression and upregulation of Bcl-2 mRNA expression,these offered further theoretical evidence for VEGF on prevention of restenosis after PCI.