An Experimental Study About The Correlation Between Hippocampal Plasticity And Epileptogenesis Of The Temporal Lobe Epilepsy

Objective: To investigate the effects of the hippocampal plasticity on epileptogenesis of the temporal lobe epilepsy and to study the correlation between them.Methods: Our research includes three parts: (l)In this study, adult Sprague-Dawley rat chronical seizure model was firstly established following status epilepticus induced by lithium-pilocarpine, this model is similar to the major character of human temporal lobe epilepsy. Afterwards we investigate the spontaneous seizures every day, record and analyse. We use the opticmicroscope and electronmicroscope to observe the morphologic character of hippocampal plasticity such as neuronal death and mossy fiber sprount dynamicly and quantitative analysis it by t-test. (2)With the immunohistochemistry method ,We detect the expression of the N-cadherin in experimental group and compare group and t-test, bivariate correlate evaluation were made in order to find the relationship between hippocampal plasticity and expression of the N-cadherin. (3)Record the electroencephalogram of the SD rat dynamically.We use extracellular microelectrode to recorde the epileptiform burst in the CA3 area of the hippocampus in TLE rat slices. We use t-test, bivariate correlate evaluation and partial correlate evaluation to analyse the relationship between hippocampal plasticity and epileptogenesis of the temporal lobe epilepsy.Results: Lithium-pilocarpine induced SD rat model includes acute period, latent period and chronic period. Spontaneous recurrent seizures can be observed during the chronic period.we can study the epileptogenesis of the temporal lobe epilepsy through this rat model because the clinical characters of this one is similar with that of the human being. The morphologic character of hippocampal plasticity such as neuronal death and mossy fiber sprount and synapse reformation emerged step by step. Expression of the N-cadherin in experimental group increased gradully accord to the time. The expression of the N-cadherin is correlated with the neuronal death negatively. Spontaneous epileptiform burst in the CA3 area of the hippocampus in TLE rat slices were recorded. The neuronal death is correlated with the epileptiform burst negatively.But the correlation between neuronal death and epileptiform burst dimissed when we avioded the influence of the the expression of the N-cadherin. The expression of the N-cadherin is correlated with the epileptiform burst.Conclusion: Lithium-pilocarpine induced SD rat model can be used to study the epileptogenesis of the temporal lobe epilepsy because the clinical characters of this one is similar with that of the human being.Neural plastic change can be oberserved during the chronic period in the rat model. The expression of the N-cadherin is correlated with the neuronal death negatively and may participate the change of the Neural plastic. Spontaneous epileptiform burst in the CA3 area of the hippocampus in TLE rat slices can be recorded. The neuronal death is correlated with the expression of the N-cadherin negatively and its influence on epileptiform burst were given through plasticity change,that is tosay there is no correlation between them. The expression of the N-cadherin is correlated with the epileptiform burst.