Study Of Chinese Medicine On TLR9 Of RGH Patient And TLR9,IFNα Of Mouse That Infected With HSV-2

ObjectiveThe Recurrent Genital Herpes(RGH)is a kind of frequently Transmitted Sexual Disease(TSD)mainly caused by simplex herpes virus-2(HSV-2).RGH is recurrent and difficult to cure,therefore,brings about great pressure to the patients and their families.The patients will have low quality of life and suffer from financial difficulties caused by the persisting effect of sexual disease.Pregnant women infected with genital herpes would suffer from abortion and neonatal deaths.A patient frequently infected with genital herpes risks higher chance to have womb cancer,penile cancer,prostate cancer and AIDS.HSV infection increases the risk of HIV infection,in which HSV-Ⅱacts as Cofactor for HIV,thus catches more and more attention.Toll-like receptor(TLR),the pattern recognition receptor,which enables the body to recognize the Microbial pathogens and activates cells to produce immune cells and participate in the recognition of pathogen associated molecular patterns (PAMPs)which are at the surface of immune cells,thus induces natural immune response of the body.TLRs have an essential effect on the immune system because TLRs can regulate innate immune as well as involving directly in the launch of acquired immune response.TLR9,mainly contained in B-cell and plasmacytoidpredendritic cells(PDCs),can specifically identify the CpG motifs(CpGDNA)which is rich in the DNA of bacteria and viruses,therefore, activates NF-κB,AP1 and IRF-7 to release TNF-a,IL-6,IL-8 and other inflammatory mediatory mediators and typeⅠinterferon(IIFN-s/β)to mediate acute inflammatory response and play an antiviral effect,through MyD88-dependent signal transduction pathway activated by NF-kB,AP1 and IRF-7 and other transcription factors and protein Kinase.Available evidence shows that Traditional Chinese Medicine(TCM)can improve the Anti-infection of the immunity in a variety of ways._It is expected that TCM improves the immune system to identify and kill pathogens,and induce more effective regulation of the immune defense response,which is closely related to LR and associated with the signal molecules.Thus,searching for efficient but low cost of antiviral drugs from the precious Chinese medicine resources has realistic and social significance.The purpose of this paper is to take TLR9 as a testing target to observe the function of the Chinese Anti-virus Medicine Capsules(CAMC)on the patients with recurrent genital herpes,and to observe the impact of the CAMC on TLR-9 and IFN-a in the cells of the mice infected by HSV-2 virus.Preliminary Study on the pathogenesis of RGH and mechanism of anti-viral medicine capsules will offer a new method and way for the future clinical treatment and experimental research of RGH.Method1.Clinical Research:Select 30 RGH patients as test target,comparative, compare TLR9 between patients before treatment and three months after treatment,at the same time,compare the average frequency of relapse(times/3 month),Lesions healing time between before treatment after treatment.2.Experimental Study:Divided into two parts,the first part feed the C57BL /6 mouse with number 2 Anti-virus capsules 5 days ahead of schedule,after 12 hours and 7 days,respectively get the mouse,s blood and vaginal organizations to test TLR9 and IFNα.At the same time,do a biopsy of vaginal organizations.PartⅡfeed the C57BL/6 mouse number 1 Anti-virus capsules after attack vagina with HSV-2 virus.After 12 hours and 7 days,respectively get the mouse,s blood and vaginal organizations to test TLR9 and IFNα.At the same time,do a biopsy of vaginal organizations.Results1.The frequency of recurrence reduces significantly after treatment than before treatment.The time of Lesion healing was significantly shorter. (Compare with before treatment,P＜0.05,there was a significant difference).The difference was not significant between 6 month after treatment and 3 month after treatment.(P＞0.05)2.TLR9 in patients before treatment were more Statistical significant than normal.There was not a significant difference for TLR9 levels in patients after treatment and before treatment.3.Experimental studies of the prevention trial show that:feed mice with number 2 Anti-virus capsule in advance,after Virus attacking,there was no significant statistical difference that TLR9 levels in the prevention of group 2 and in Positive control groupl.There was also no significant statistical difference comparing with the prevention of group 1.But it was statistically significant difference to compare these 3 groups with the normal group.4.Experimental studies of treatment trial show that:Levels of TLR9 in Lymphocyte show that:it was no statistically significant difference to compare the treatment Group 1 with the control group1.it was no statistically significant difference to campare the treatment group1 with the treatment group2.Compare with average lewes of vaginal tissue:it was statistically significant difference to campare the treatment group2 with the normal group;the positive group1 with the positive group 2;the treatment group 1 with the treatment group 2;the positive group 1 with the normal group.But it was no statistically significant difference to compare the treatment group 1 with the treatment group 2..5.IFNαDetection in two groups experimental Study shows that:in the prevention trial the IFN-αlevel in four groups were no statistical difference. However,from the terms of content,the prevention of group 2 was higher than the positive control group1,the prevention group and the prevention group were higher than the normal group.In the treatment trial IFNαlevels in four groups have statistical difference,there was significant difference to compare treatment Group 1 with positive control group 1 and compare treatment Group 1 with the normal group,but there was no difference to compare the treatment group 1 with the treatment group 2.Conclusion1.take TLR9 testing as to be evaluation of anti-virus capsules,we can find that compare with TLR9 levels between patients before treatment and normal people was Statistical significant,and there was not a significant difference for TLR9 levels in patients after treatment and before treatment.That mean Anti-virus capsules could be maintained TLR9 level.We researched Anti-virus capsules in the role of immune regulation from the natural immune area.the frequency of recurrence reduce significantly after treatment than before treatment And the time of Lesion healing was significantly shorter,that means Anti-virus medicine capsules,could improve clinical efficacy,reduced relapse frequency,shortened healing time lesions,alleviated the suffering of patients.2.Experimental studies show that:after the animals were attacked by HSV-2 virus,TLR9 caused the innate immune HSV.Prevention trial showed that:feed mice with Chinese herb in advance,the small molecule of Chinese medicine may be combination with some activities of TLR9.Treatment trial showed that:it switched on natural immune when the virus was attacking after the Chinese medicine treated,it basic maintain natural immune.Prevention and treatment trial showed that:when the natural immune was switched on,the level of IFNαthe end of its signal transduction don,raised,it was possible startedγof Type 1 of IFN or other Cytokines..