The Intervenient Study On Acupuncture In The Model The Guinea Pig Trentment Of â…£ Allergic Skin Disease

Introduction:In recent years,the incidences of allergic diseases are on the rise all through the world,Of all,allergic skin diseases are ranked on the top so that they have appealed To more and more researchers on how to apply effective therapies to prevent and cure them. Tradjtional Chinese Medicine has so far thrown light on the theraPies,harvesting Comfortable efficacy on the allergic skin diseases by way of differentiation of symptoms and signs.Experiment prove,acupuncture curingⅣmetamorphosis have been Proofed to inhibit the generation of allergia by many means,showing Priorities in general Pharmacological action to Western medicine.Therefore,Some traditional acupuncture cure,especially research acupuncture mechanism ofⅣmetamorphosis through experiment study,and open another door for the prevention and treatment of diseases of the sort as soon as they are studied by using modern experimental means.Objective:To observe the effects of acupuncture on the models ofⅣmetamorphosis in guinea pigs,and explore its action mechanism.Methods:1.Establishment ofⅣallergic skin disease of guinea pigs model,and observe strong and weak of guinea pigs inflammation reaction in acupuncture treatment.guinea pigs feed one suitable week after buy it,45 healthy guinea pigs,male and female of half and half,15 of a group,3 stochastic groups,guinea pigs were unhaired at the abdominal part with an area of 3 cm~2 and allergized by aPPlying 5%2-4-DNCB tow the week,Allergiawas in duced by applying 0.1%2-4-DNCB(every one four week)evenly on the back of guinea pigs left ear on the 48 hours,and after 72 hours to settle experiment.model groups, To compare groups,model groups and acupuncture groups,the compare groups do not anything experiment,model groups do establish guinea pigs model,acupuncture groups do acupuncture treatment after choosing acupuncture points,acupuncture points:QueChi, XueHai and DaZhui.Make use of G-6805 electric acupuncture treat instrument,5-10Hz electromagnetic wave,make softly vibration around acupuncture points,3-5V,acupuncture one time every day,and 30 minutes at every turn,at a distance from treatment 3 day,10 days 1 period of treatment.Killing guinea pigs when draw materials,The differences of body weights of mice were measured using an electronic scale before and after the exPeriment,the thickness of middle Part of bilateral ears Was measured using external diameter micrometer and the tissues obtained from middle Part of bilateral ears with a Puncher were weighed to calculate the differences of weights between the bilateral ears,observe strong and weak of guinea pigs inflammation reaction in acupuncture treatment.2.The mononuclear and Polynuclear cells were counted out under an electronic microscope,and explore influence of acupuncture moxibustion forⅣallergic skin disease.Through making guinea pigs model after 48 hours,The tissues obtained from left ears of the guinea pigs were by HE stained to Pathological examination,observe pathology changment,and observe pathology change every groups under an electronic microscope.3.Observe Th1/Th2 Cellular consistency level on the models ofⅣmetamorphosis in guinea pigs through immunity methods,and measure Th1/Th2 Cellular consistency level of TNF-α,IFN-γ,及IL-2,IL-4,IL-10.Guinea pigs feed one suitable week after buy it,45 healthy guinea pigs,male and female of half and half,15 of a group,the compare groups do not anything experiment,model groups do establish guinea pigs model,acupuncture groups do acupuncture treatment after choosing acupuncture points.Tow hours after induction,the heart of guinea pigs were Picked for collection of blood,pour into PE tube though moving liquid tube,make centrifugal machine 1500r/min every 15 minutes,folsmalled by separation of blood serum, Then the expressions of cell level guinea pigs Peripheral blood were detected by ELISA.4.Observe CGRP consistency variation of blood plasma at the model the guinea pig trentment ofⅣallergic skin disease,guinea pigs feed one suitable week after buy it,45 healthy guinea pigs,male and female of half and half,15 of a group,3 stochastic groups,the compare groups do not anything experiment,model groups do establish guinea pigs model, acupuncture groups do acupuncture treatment after choosing acupuncture points.Tow hours after induction,the heart of guinea pigs were Picked for collection of blood,inpouring the testtube which have 10%EDTA and the restraining peptide enzyme though moving liquid tube,enzyme,mixed them equably,centrifugate the plasma 10min under the condation 40℃,1500r/min,measure the CGRP consistency by RIA method.Results:1.The difference of thickness and weight prove inflammation level from macroscopic in 3 groups guinea pigs,inducing the thickness difference in front and behind of left ear, inducing the thickness difference of the left and right ears and inducing the weight difference in front and behind of left ear,were batter than compare groups(P＜0.05).There was statistical difference between thickness and weight of ears.so this prove restrain intensity for inflammation level,the divergence is batter low,effect is batter strong.2.In pathology changes,compare groups prove no obvious changes for surface skin, genuine leather and cartilage,for model groups,surface skin hyperplasia,acanthosis, hyperkeratosis,parakeratosis,cells dropsy papillaryepidermiccells,at genuine leather surface,phlogosis infiltration,a large number lymphcell,a few neutrophilecell and fibroblast,express chronic eczema,for acupuncture groups surface skin mild hyperplasia, mild acanthosis,at genuine leather surface,mild phlogosis infiltration,among which lymphatitis monocyte neutrophilecel fibroblast,is obvious reduce than model groups.form pathology angle,it prove that acupuncture have batter effect forⅣallergic skin disease.3.The Th1/Th2 Cellular consistency change on acupuncture in the model the guinea pig trentment ofⅣallergic skin disease,the consistency of compare groups and acupuncture groups is very high for IL-2,There was statistical difference between model groups and compare groups,the consistency of compare groups and model groups is very high for TNF-α,There was statistical difference between model groups and compare groups,the consistency of compare groups and acupuncture groups is very low for IFN-γ,IL-10,There was statistical difference between model groups and compare groups,acupuncture groups,the consistency of compare groups and acupuncture groups is very high for IFN-γ,/IL-10,There was statistical difference between model groups and compare groups,acupuncture groups,the consistency of IL-10 is most low for acupuncture groups,to IL-2,IFN-γ,,IL-10 and TNF-α,the balance of Th1/Th2 cellular factor have notable transform.particularly decrease the ratio of IFN-γ,/IL-10.4.Observe CGRP consistency variation of blood plasma at the model the guinea pig trentment ofⅣallergic skin disease.There was statistical difference between model groups and compare groups,acupuncture groups,this show the consistency of CGRP is low before model,for acupuncture regulate humoral immunity of neuropeptide,it accord with raising consistency of guinea pigs CGRP.Conclusions:Acupuncture have obvious effects ofⅣmetamorphosis in guinea pigs.