Pungent Stasis Contact With The Insects Circulation Network Of Newborn Rats With Cerebral Infarction Vascular Contrast

Objective:"Huayu Tongluo" collateral disease in the medical treatment of an effective treatment,it is an important aspect of the new Health and bleeding Tongxinluo to Tongluo.This is the treatment of angiogenesis is similar to Italy.In recent years, angiogenesis therapy in peripheral occlusive disease and myocardial ischemia,such as the treatment of ischemic disease in the success and is the current treatment of collateral disease one of the main research directions.Therefore,this issue in the preliminary studies on the basis of further observation Xinwei Huayu Tongluo law and the Tongluo Huayu Insects Act of cerebral infarction(brain Tongxinluo Stagnation)rat brain penumbra area VEGF the impact of molecular And two levels of cells,genes and proteins two aspects,the two comparative studies of Chinese medicine for the collateral disease Huayu Tongluo for the initial treatment of the experimental data and theoretical basis.Method:1.The theoretical research literature by finishing,combined with clinical experience in the way.2.The experimental set up six groups:normal,model,Xinwei low-dose group,Xinwei high-dose group,Insects low-dose group,Insects high-dose group.To copy the thread of rat model of cerebral infarction.The treatment group were given Xinwei Huayu Tongluo side,the side Insects Huayu Tongluo gavage and the rest of the group were given saline equivalent gavage.7 days after delivery,the following test:RT-PCR method to observe the expression of rat brain VEGFmRNA the situation to immunohistochemistry test Xinwei Huayu Tongluo law and the Tongluo Huayu Insects Act of rat brain VEGF protein and CD31 antigen positive impact of the MVD.Results:1."Huayu Tongluo" law passed Quchu bleeding,clear the meridians;smooth transfer of gas,the flow of qi and blood;nutrition organizations,to promote the three freshmen to achieve stasis of Health and new blood,new Tongxinluo and the purpose of the new.2.the dose of VEGF mRNA expression of both enhanced,in particular the high-dose group Insects,in particular,significantly enhanced.Xin Wei Huayu Tongluo Huayu Tongluo Insects and each group can increase the rat brain cells of the VEGF protein expression,and the normal group there was a significant difference(P＜0.01); compared with the model group was significant And very significant difference(P＜0.05,or P＜0.01).Xinwei especially low-dose group and low-dose group Insects are significant differences(P＜0.05);Xinwei high-dose group and high-dose group Insects have a very significant difference(P＜0.01).At the same time,Xinwei low and high dose group there were significant differences(P＜0.05);Insects low and high dose group have a very significant difference(P＜0.01).Model,Xin Wei Huayu Tong Luo Huayu Tongluo Insects and each group of MVD(MVD)than the normal group had increased,Xinwei high-dose group,high-dose group increased Insects particularly evident,especially high-Insects The most obvious increase in dose.Conclusion:Xinwei Huayu Tongluo law and Insects Act Huayu Tongluo thread can be enhanced by rat brain infarction CD31 positive MVD,may be raised through the vascular endothelial growth factor(VEGF)gene and protein expression to Implementation of the role of angiogenesis,and Insects Act Huayu Tongluo the role of stronger-than-Xin Wei Huayu Tongluo law.