Clinical Significance Of The Change Of CD4~+CD25~+ Regulatory T Cells In Patients With Multiple Organ Dysfunction Syndrome

multiple organ dysfunction syndrome (MODS) is defined by that patient with severe acute injuries may occur two or more organs severe dysfunction even failure sequentially or simultaneously in short time (>24h). MODS is the most capital cause of death in such disease as severe infection, trauma, and heavy shock et. MODS is a common, complex, threatened serious illness. MODS has a high mortality rate ,and is the main cause of death in intensive care unit.In the past 40 years, people has made more efforts to research MODS and made some achieveness in pathogenesis, organ damage, clinical score,causal prophylaxis and combined therapy.But there are too much matter to tackle now.My study is to explore the significance of the dynamic change of CD4~+ CD25~+ regulatory T cells(Treg), inflammatory and antiinflammatory cytokines.To explore their raletives, to explore their associatives with prognostic.We try to find a new method to prevent or treatment the disease of MODS.Methods:The frequencies of CD4~+ CD25~+ CD127- Treg were detected in 42 patients with MODS respectively 1 day and 3~7 day after in hospital and 10 healthy donors by flow cytometry labeled with specific fluorescent antibody, such as anti- CD3 (PerCP5), anti-CD4 (PE–CY5), anti-CD8 (PE), anti-CD4 (PerCP5), anti-CD25 (FITC) and anti-CD127 (PE). Simultaneously get blood from vein to detect the concentration of IL-2, IL-6, IL-8, IL-10, IL-4, TNF-аby ELISA. Results:The frequency of CD4~+ CD25~+ CD127-Treg in the patients with MODS in 1 day was significantly higher than that of those in 3~7 day, and they were all higher than that of healthy donors (P<0.05). The frequential of CD4~+ CD25~+ CD127-Treg of survival group in 1 day is higher than that of death group (P<0.05). They has no significantly differences in 3~7day. The concentration of IL-2, IL-6, IL-8, TNF-аof MODS patients is higher than that of control group in 1day and 3~7day (P<0.05). The concentration of IL-10, IL-4 in MODS patients is decreased gradually in 1 day and 3~7day (P<0.05), It is higher than that of control group in 1 day, but it has no differences from that of normal control group in 3~7day. The concentration of IL-2,IL-4,IL-6 is positive relatively with the APACHEⅡscore of MODS patients. The concentration of, TNF-a, IL-10 and Treg has no relativity with the APACHEⅡscore of MODS patients. Conclusions:The frequency of CD4~+ CD25~+ Treg increases in patients with MODS. That may surpress the process of MODS. The lower the frequential of CD4~+ CD25~+ Treg of MODS patients, the poorer the prognostic. The IL-2, IL-6, IL-8, IL-10, IL-4 and TNF-аpaticipate the occur and progress of MODS. The concentration of IL- 2, IL-4, IL-6 is relative with the severe degree of the disease.