| Objective: Multiple organ dysfunction syndrome (MODS) is an important topic investigated in the realm of medicine. It is a syndrome that two or more organs simultaneously or sequentially appear dysfunction even failure after severe trauma or injury, infection and burn and so on.At present, MODS has been the most common cause of death in the intensive care units (ICUs) with high morbidity, and traumatic MODS is also one of the common causes in forensic practice.The mechanism underlying traumatic MODS has been extensively studied, however, up to now changes and role of lung antioxidant ability in lung dysfunction even failure was still unclear. In this study, the rabbit model of MODS was developed through compression hindlimbs where muscle is abundant, accompanied with lipopolysaccharide ( LPS ) administration via vein. Morphological and functional changes of liver, kidney and so on were measured to clarify the occurence of MODS.Lung antioxidant ability and lipoperoxide were observed to elucidated the role of oxidative stress in pulmonarydysfunctions, and ultrastructural changes of pulmonary and thoracic arterial endothelial cells were observed with electron microscope, to provide morphologic basis of endothelium impairment and investigated its pathogenesis of traumatic MODS in forensic medicine and direct therapy in clinical medicine.Materials and methods: Forty healthy New Zealand white rabbits of either sex, weighting 1.8-2.2kg were used in the experiment. The rabbits were free of chow, access to water freely 24h before experiment. Establishment of rabbit model of MODS was developed as follows: The hindlimbs were compressed with 22.5kg standard heavy object for 3h, releasing compression for 30min, then continuing being compressed for 30min twice. After compression, LPS(2mg/kg b.w.) was injected via the vein.The normal group is sham animals.Rabbits were divided into 4 groups: LPS and compression for lOh ( C+LPS lOh) group, LPS and compression for 2h (C+LPS 2h) group, simple compression(C) group and sham group, and each group had 10 rabbits.In experiment group, blood samples were taken for determining the levels of alanine aminotransferase (ALT), aspartatae aminotransferase (AST), blood urea nitrogen(BUN) and creatinine(Cr), lung tissues for determining the levels of malondialdehyde (MDA) contents and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase(GSH-Px) activity and total antioxidant capacity (TAG)after experiment.Animals were put to death and lung, liver, kidney tissues and so on were fixed for microscope, and segments of pulmonary and thoracic arteries were taken and fixed with 2.5 and 4 percent of glutaraldehyde solutions for elecron microscope.The data were shown with x+s, with POMS software used for statistical analysis.Results: 1.Changes of liver and kidney functions: As compared with sham group (35 + 9.66U/L) , ALT values in C, C+LPS 2h and C+LPS lOh group significantly increased, and was 150.90+ 39.09U/L (PO.01 vs sham group), 72.12 +12.38U/L(P<0.01 vs sham and C group), 196.71 +78.78U /L (P<0.01 vs sham and C+LPS 2h group) , respectively.As compared with sham group (15.62 + 5.32U/L) , AST values in C, C+LPS 2h and C+LPS lOh group obviously increased, and was 164.60+85.72U/L (PO.01 vs sham group), 123.62 + 31.14U/L(P<0.01 vs sham group), 334.43 +148.13U/L (P<0.01 vs sham, C and C+LPS 2h group) , respectively.As compared with sham group (6.49+2.55mmol /L) , BUN values in C, C+LPS 2h and C+LPS lOh group markedly increased, and was 12.85 + 2.5 lmmol/L(P<0.01 vs sham group), 10.87+3.16mmol/L(P<0.01 vs sham group), 19.18+4.82mmol/L (PO.01 vs sham, C and C+LPS 2h group, respectively) .respectively.Cr value in C, C+LPS 2h and C+LPS lOh group was82.20+21.50nmol/L, 83.12+14.01umol/L, 146.50+46.23 umol/L, respectively, and significantly increased in C+LPS lOh group and had significant difference (P<0.01) compared with sham group (80.75+14.70umol /L) .2.Wet to dry weight (W/D) ratio and water contents in lung, liver and kidney2.1...
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