Studies On The Technology For Separation And Preparation Of Lignan Compounds From Fructus Forsythiae And The Pharmacodynamic Activities

Chinese medicine modernization research has been a hot subject in medicine field, while it is an important content that separating active components from Chinese medicine by modern technique. According to the pharmacodynamic activities, using the modern techniques of Microwave assisted-semi bionic extraction, macro porous resin seperation and column chromatography seperation, systematically study the sepertion art of lignan components, and getting an industrial art and related parameter;exploring the biological activities of lignan components on Anti-aging, oxidation resistance and protection of brain injuryby in vitro and in vivo experiments ,and get a series meaningful achievements.1. Studies of different extraction arts of lignan components of Fructus Forsythiae.Extracting lignan components of Fructus Forsythiae by traditional Heat Reflux extraction, definite optimize parameter of extraction art. The process of Heat Reflux extraction is stable, and needs common apparatus, the condition is adapt to most of Chinese medicine manufactures at present. Study dynamics of Heat Reflux extraction under the optimize condition, the result shows that extraction process is an internal spread controlling one, and the extraction apparent activation energy is 19.74 kJ/mol, extraction dynamics equation as following:Extracting lignan components of Fructus Forsythiae by microwave assisted-semi bionic extraction which imitated gastrointerstine surrounding in human, the results show that the extraction efficiency of microwave assisted-semi bionic extraction is higher, extration yield of lignans reach 87.5% under the optimic condition within 20 min and twice extraction, which means lower energy and higher yield, and has a good future.2. Study on purification of lignan components of Fructus Forsythiae. Preparing 93.5% lignans of Fructus Forsythiae by solvent extraction-macro porous resin seperation-column chromatography seperation.Selecting X-5 macroporous resin to purifying lignans of Fructus Forsythiae. The conditions are as following, temperature is 25 ======, content of lignans in solvent for column is 1000mg/L, absorbing velocity is 2BV/h, total volumn for absorbing is 8 BV;desorption process are as follow, water rinse at first, then by 80% ethanol and controlling the velocity for 1BV/h, getting 82.5% lignans after using 3BV ethanol under the optimize condition. This art is simple, using less solvent, causing less pollution , reducing cost, being adapt to industralization.According to study for the function of X-5 macro porous resin, determining the absorbing process of X-5 macro porous resin is physical absorbtion of single molecular, and it is spontaneous absorbtion, and it is easy to absorb under lower temperature.It can be imitated by Langmuir absorption isothermy equation that X-5 macro porous resin absorbs lignans process.3. Studying the oxidation resistance of Fructus Forsythiae extraction, and find its activity is better than anisole synthesized by chemical way, therefore, Fructus Forsythiae extraction can be developed as a new antioxidant. Studying the anti-aging and anti-oxidation of Fructus Forsythiae extraction, the results show lignan components can get rit of all kinds of free radicals which cause aging.4. Studying the biological activity of lignan components on protection of brain injury. Comparing with neuro-function of experimental rats in lignan components treatment group, control group, sham group after brain ischemia/reperfusion, measuring the content of erythrocuprein (SOD) , malondialdehyde (MDA) and calcitonin gene-related peptide (CGRP) in plasma of mice, observing the ET-3 and GFAP positive cell in hippocampus, results show that lignan components can decrease lipid oxidation and expression of ET-3, and decrease protein degeneration and membrain injury, and protecting ischemia/reperfusion injury. So we can conclude that it is one of important pathological processes in ischemia/reperfusion mice models that free radical formation and lipid peroxidation occur. Lignan compounds in fructus forsythiae can protect neurons in ischemia/reperfusion mice models from injury, and its antioxidation mechanism is perhaps related with the expression of calcitonin gene-related peptide.