| Objective:To test if nephritis Xiaobaifang decreases proteinuria level in experimental animals and investigate its mechanism by biochemical and molecular biological analysis to provide the basis for its clinical application.Methods:We utilized a rat model in which nephritis is induced by one-time tail vein injection of doxorubicin.Animals were divided into five groups,namely the naive control group,model control group,Lotensin group,low-dose nephritis Xiaobaifang group and high-dose nephritis Xiaobaifang group.Seven weeks after treatment,we detected rat urine creatinine,serum BUN,Scr,Alb,urineβ2-MG(by ELISA),proteinuria, COL-Ⅳand LN expression in kidney(by immunohistochemical staining), and nephrin mRNA and CD2AP mRNA expression in kidney tissue(by RT-PCR).Results:At the end of 7-week observation,the ratio of proteinuria / urine creatinine values(P＜0.05)were reduced in animals from all treatment group with doxorubicin-induced nephritis,and the high-dose nephritis Xiaobaifang treatment had the most significant effects(P＜0.05);the urineβ2-MG level(P＜0.05)was also decreased in all treatment group,and the high-dose nephritis Xiaobaifang treatment had the most significant effects (P＜0.05);the serum albumin concentration(P＜0.05)was increased in all treatment group;and high-dose nephritis Xiaobaifang treatment had the most significant effects(P＜0.05);the expression of both COL-Ⅳand LN in the kidney were decreased in all treatment group(P＜0.05),and the high-dose nephritis Xiaobaifang treatment had the most significant effects (P＜0.05);the treatment by nephritis Xiaobaifang did not affect the kidney function in rats with nephritis(P＞0.05);the expression of both COL-Ⅳand LN in the kidney tissue were decreased in all treatment group(P＜0.05), and the high-dose nephritis Xiaobaifang treatment had the most significant effects(P＜0.05);all animals in the treatment group had attenuated foot cell damage,lower nephrin mRNA and CD2AP mRNA expression in kidney tissues,and the high-dose nephritis Xiaobaifang treatment had the most significant effects(P＜0.05).Conclusion:nephritis Xiaobaifang can reduce excretion a certain extent, decrease urineβ2-MG level in nephrotic rats and protect tubulo;It can possibly increase serum albumin level by reducing excretion;It can attenuate COL-Ⅳand LN expression in kidney tissues,so it can treat kidney fibrosis;It can improve the foot cell morphology of nephrotic rats, by which it reduced excretion;It can reduce nephrin mRNA and CD2AP mRNA expression in kidney tissues from rats with doxorubicin-induced nephritis,repair injured foot cell,then.reduce excretion.The therapeutic effects with high-dose nephritis Xiaobaifang are better than low-dose nephritis Xiaobaifang or Lotensin. |
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