WT1 Expression In Ovarian Carcinoma And The Effect Of Its Antisense RNA On Induction Of Apoptosis In Vitro And In Vivo

Objectives To study the expression of WT1 protein in ovarian carcinoma and the effect of WT1 ASODN inducing proliferation and apoptosis of SKOV₃ in vitro and in vivre.Methods Expression of WT1 and gene correlated with apoptosis were studied in all kinds of ovarian tissues and nude mouse transplanted tumor treated with Taxol. The WT1 ASODN was transferred into SKOV₃ cells.Investigate the changes in expression levels of WT1 protein and cell proliferation and apoptosis in ovarian epithelial after transfection.Results The positive expression rate of WT1 in ovarian epithelial carcinomas,especially in serous carcinomas,was statistically higher than those in other histologic subtypes.There were closed relationship between the expression of WT1 and clinical stage,histological grades,but no relationship with lymph node metastasisand ascites. Low lever of WT1 and apoptosis occurred were detected in nude mice during Taxol treatments.The growth of the ovarian cancer cell lines SKOV₃ transfected by WT1 ASODN became significantly slow and its activity reduced.Apoptosis were found in early stage.The tumorigenic ability of the SKOV₃ transfected by WT1 ASODN was decreased.Conclusion The overexpression of WT1 gene might be the biomolecular markers for the clinical diagnosis of ovarian epithelial tumors,especially serous carcinomas.WT1 may play an important role in regulate the proliferation and Apoptosis of ovarian cancer cell lines SKOV₃. WT1 will become a new diagnosis and therapeutic target in ovarian epithelial carcinoma.