| At the point of keratinocytic proliferation, angiogenesis and invasion of inflammatory cell in psoriatic lesions, the adhesion molecules, VEGF, lymphocyte-associated antigen (CLA) in lesions and peripheral bloods from patients with psoriasis were detected by immunohistochemistory, Enzyme linked immunoabsorbent assay(ELISA) and flow cytometric analysis. we investigated the modulation of acitretin to the proliferation of endothelial cell keratinocyte line HaCaT by immunohistochemistory and MTT method; we measured the variation of acitretin to the cell cycle of endothelial cell and HaCaT with the flow cytometric method, According to the tradition Chinese medicine we composed a complex herbs of"QiPiYin"to treat psoriasis, then detected the content of VCAM-1 and IFN-γby Enzyme linked immunoabsorbent assay(ELISA) before and after treating. The results showed as the following:①By using statistical analysis , the results show that the intensity of each expression: VCAM-1> PECAM-1> ICAM-1> VEGF> LFA-1> VLA-4> Mac-1. PECAM-1 is the sensitive sign of the blood vessel proliferation of dermis layer in psoriatic lesions.②Increased VCAM-1 in serum set up the sensitive and special mark of laboratory to distinguish diagnosis and treatment of psoriatic.③The results shows that in psoriatic skin lesions the expression of CLA and its ligand E-selectin was strongly positive, that in peripheral blood CLA on CD4+,CD8+,CD3+T cell from progressive phase patients was much higher than that from static phase psoriatic patients and also higher than health controls. Compared with health controls, only the frequency of CLA+CD8+T cells was increased in static phase psoriatic patients.④HUVEC reacted to acitretin treatment with a double direction of modulation. Along with the gradually increased concentrations, the endothelial cell proliferation rates were enhanced earlier with a peak effect, and then were inhibited. HaCaT reacted with an obviously inhibitory effect. In a time-course study, the effect of acitretin on cell proliferation was notable gradually.⑤HUVEC and HaCaT cell cycle modulations to acitretin were different completely. The drug induced G1-phase arrest of endothelial cell, and S-phase arrest of HaCaT.⑥The heat blood type psoriasis showed to improve sing and reduce PASI score in lesions and decline sVCAM-1 and sIFN-γcontent in serum psoriasis patient by intervened Chinese traditional herbs—"QiPiYin". Chinese Wolfberry Root-bark can promote the proliferation of HaCaT . White Mulberry Root-bark,Cortex Moutan,Areca Peel,Silktree Albizia Bark,Dictamni Cortex,Dried Tangerine Peel and"QiPiYin"can inhibit the proliferation of HaCaT. And with the increasing of concentration,the effect was enhanced.The inhibitive action of"QiPiYin"on HaCaT was the best. Further study by DNA flow cytometric analysis revealed that"QiPiYin"could alter the distribution of HaCaT cell cycle obviously. which shown that the cell percentage of HaCaT in G0/G1 phase increased and those in S phase decreased.
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