Study On The Inducement Mechanism Of Gongqing Granum Concerning Signal Transduction In Cell Apoptosis Of Human Villi And Decidua In Early Pregnancy

Objective:To investigate the inducement mechanism of Gongqing granum concerning singnal transduction of human villi and decidua cell apoptosis,which is in the early pregnancy bleeding after drug abortion.In order to provide objective basis for preventing and treating the disease by Gongqing granum,furthermore,enrich the scientific connotation of the TCM theory that "there are more fragility and blood stasis after production".Methods:In the modern TCM literature study,a database of recipes and herbs was founded with computer technology.Then analyzed the therapia rule from the point of frequency analysis,factor analysis and varable clustering analysis.In the clinical experimental investigation,the selected 240 patients were divided into 4 groups by random.They were group of Gongqing,group of Qianzhi,group of drug abortiona and group of induced abortion respectively.We observed the clinical curative effect of the 4 groups,at the same time,we collected the specimen of the 4 groups.Then we observed the ultrastructure of the specimen and detected the expression of apoptosis rate(AR) and correlative indexs of singnal transduction of cell apoptosis,such as FasmRNA,FasLmRNA, Fas,FasL,PKC and intracellular calcium concentration([Ca²⁺])i,furthermore,we analyzed the relativity of the indexs.Results:The TCM literature study showed that the main pathogen of bleeding after drug abortion were blood stasis,fragility and heat,which offered the philology evidence of Gongqing granula preventing and treating bleeding after drug abortion.The clinical experimental investigation showed that the clinical curative effect of Gongqing granum was obviously better than other groups(P＜0.01,P＜0.05).The observation of ultrastructure indicated distinct change of apoptosis.Compared to the other 3 groups,the expression of AR,FasmRNA,FasLmRNA,Fas,FasL and([Ca²⁺])i of Gongqing group were obviously higher than the other 3 groups(P＜0.01,P＜0.05),while the expression of PKC of Gongqing group was obviously lower than other 3 groups(P＜0.01, P＜0.05).Results:Mifepristone can affect the singnal transduction of cell apoptosis caused by singnal transduction Fas/FasL,PKC and Ca²⁺.This may be one of the mechanisms of drug abortion.Blood stasis,fragility and heat are the main reasons of bleeding after drug abortion.Gongqing granum can adjust the signal transduction caused by Fas/FasL,PKC and Ca²⁺,consequently induced the cell apoptosis of human villi and deciduas in early pregnancy.By doing so,Gongqing granum can prevent and treat abnormal uterus bleeding after drug abortion.The effect of clinical curative and adjustion of apoptosis singnal transduction of Gongqing granum are obviously better than Qianzhi and onefold drug abortion.