Study On Anti-Metastasis Mechanisms Of Reinforcing Qi And Nourishing Yin Through Angiogenesis Inhibition Effect And Gene Expression Profile Analysis

Objective To explore the mechanisms of Reinforcing Qi and Nourishing Yin in inhibiting tumor metastasis. Methods By duplicating tumor spontaneous and experimental models separately and using immunohistochemical technique and gene chips, some indexes were observed in tumor-bearing mice treated by Reinforcing Qi and Nourishing Yin, including tumor inhibition rate. Inhibition rate of metastasis, changes of hemorheology, effects on tumor angiogenesis and the expression of tumor metastasis-related genes. Results Reinforcing Qi and Nourishing Yin could inhibit tumor spon-taneous and experimental haemato-genous metastasis and lymphatic metastasis. Hemorheology showed that it could improve blood high viscosity in tumors. Experiments of CAM indicated that this decoction could reduce the expression of VEGF and MMP-2 in tumor cells, thus inhibiting tumor angiogenesis. The analysis of gene expression spectrum revealed that it could regulate multiple target genes such as tumor filtration and metastasis, signal transduction, cell proliferation, apop-tosis and metabolism-related genes. Conclusion It is demonstrated that Reinforcing Qi and Nourishing Yin has the effect of anti-tumor metastasis. Improving hemorheological conditions, reducing the expression of VEGF and MMP-2 and regulating the expression of tumor metastasis-related genes may be the key mechanisms.