Effects Of Exercise-induced Ftigue On The Expression Of GDNF,GFRα-1 MRNA And Protein Of Mice' Hippocampus And The Regulation Of Tonifying Qi Nourishing Blood And Reinforcing Kidney Recipe

Aim:To investigate GDNF and it's receptor GFRa-1 expression changes in mice hippocampus after exercise-induced fatigue and the effects and central mechanism of Tonifying Qi Nourishing the blood and Reinforcing Kidney Recipe on exercise-induced fatigue.Methods:100 healthy mice were randomly divided into control group, model group, the group of western medicine and the group of Chinese medicine, and established the model of exercise-induced fatigue through swimming without load of increasing interision.The lever of GDNF,GFRa-1 gene and protein expression, the concentration of 5-HT,DA,MDA, the activity of SOD,GSH-PX,iNOS were measured by Real-time Fluorescent Quantitative PCR,immunohistochemical method,high performance liquid chromatography (HPLC) and spectrophotometr method.Results:The mRNA and protein expression levels of GDNF and GFRa-1 were significantly higher in model group.5-HT, MDA levels were significantly lower in the group of Chinese medicine, furthermore DA levels, SOD activities, GDNF,GFRa-1 mRNA and protein expression levels were significantly increased in the group of Chinese medicine.Conclusion:Exercise-induced fatigues have increased the lever of GDNF and GFRa-1 mRNA and protein expression, which suggests that both GDNF and it's receptor GFRa-1 may involved in the neurobiological modulation for exercise-induced fatigue. Tonifying Qi Nourishing blood and Reinforcing Kidney Recipe may play a neuroprotective role in delaying or eliminating the occurrence of exercise-induced fatigue by reducing 5-HT, MDA content, increasing DA content, SOD activity, and GDNF,GFRα-1 mRNA and protein expression levels.