Produced In Sichuan Angelica Coumarin Separation, Quality Control And Transdermal Research

Angelica dahurica(Fisch.ex Hoffm.)Benth.et Hook.f.var.formosana (Boiss.) Shan et Yuan is a genuine staple herb of Sichuan,has the function of dispel Wind and remove Dampness,dredge the orifices to relieve pain,subdue swelling and evacuate of pus.It is ofen used to cure all kinds of pain.In this reaserch,the seperation process of active components in Angelica dahurica(Fisch.ex Hoffm.)Benth.et Hook. f.var.formosana(Boiss.) Shan et Yuan of treating hemicrania is studied. After that the quality standard and outside-body transdermal characteristics of the effective extraction products was researched.First,content of coumarin was chosen as indexe,single factor and orthogonal design were used in selecting the optimum extract and separate conditions.After the best technologic parameter was ascertained,pilot experiment was finished.Under the best condition,10 batches of extraction products were made.The content of coumarin in extraction products can be above 55%.It is proved that the technique of preparation was reasonable and practical.Pharmacodynamic experiment was used to identify the extraction products is active for curing hemicrania.Then the active compound was separated by the sillaca gel chromatography.7 constituens were separated from the active extraction products.The degree of purity in two of those constituents were identified by HPLC,TLC,determination of melting point.The structures of 5 constituens else were confirmed by ¹H-NMR,¹³C-NMR,MS.The above reseach can offer evidence for the active components of Angelica dahurica(Fisch.ex Hoffm.)Benth.et Hook.f. var.formosana(Boiss.)Shan et Yuan in treating hemicrania.In order to make sure that active extraction products is contronllable, stable,research on quality standard of active extraction products was carried out.The research was as follows:the general items,TLC to identify active extraction products,UV-VIS and HPLC to determine the content of coumarin and imperatorin.RP-HPLC fingerprint for quality assessment of extraction products from Angelica dahurica(Fisch.ex Hoffm.)Benth.et Hook.f.var.formosana(Boiss.) Shan et Yuan is the first time to reaserch..Improved Franz diffuse pool adopted to study the outside-body transdermal characteristics of active extraction products from Angelica dahurica(Fisch.ex Hoffm.)Benth.et Hook.f.var.formosana(Boiss.) Shan et Yuan the first time.For the purpose of improving the transdermal pervasion quantity of the drug,several common permeation promoters were filtered,and the experimental results indicated that the coumarin of Angelica dahurica(Fisch.ex Hoffm.)Benth.et Hook.f.var.formosana (Boiss.) Shan et Yuan itself had good penetrability,whose transdermal velocity can reach about 4μg/cm²Â·h.Adding permeation promoters as azone,oleic acid and borneol,can increase its penetrate velocity, especially 2%borneol.It can improve the penetrate velocity to about 26.905μg/cm²Â·h.It can lay a foundation for reseach safe,effective, steadily and controllable transdermal delivery preperation.