Progress Of The Disease And Yang Disease Of Diabetic Retinopathy Machine And Its Metabolomics Research

Objective:To explore the effection of Yang deficiency in the disease aggravation based on studing characteristics of the Zheng of chinese medicine with diabetic retinopathy development and identify the metabonomics of DR and Yang deficiency in order to explain the mechanism of action for DR progress and guide clinical treatment.Methods:Designed by multicentre,the clinical research was carried out in 9 hospitals all over the country.Totally,603 cases with type 2 diabetes were collected and its information database including the special questionnaire and experimental tests was recorded and established,then analyzed DR characteristic of the Zheng depending on the three different stages and the risk factors of DR occurrence process by data mining methods.Based on the former study results,we screened 89 DR patients and 30 normal subjects.These patients were divided in 2 groups as the difference of Yang deficiency.(yes\no). All subjects were collected blood serum which would determine the metabonomics changes by applying the GC\TOFMS technology.The metebonomics finger prints were analyzed by using the OPLS and OSC-PLS method in order to gaining distinction between the different groups and potential biological markers.Results:The whole distribution characteristic showed that symptoms became diversity and complication with the development of DR from orders of Chinese symptoms and Zheng frequency analysis results,and both Qi and Yin deficiency were basic performance and Qi weakness gradually became worse with the DR condition development and Yang deficiency was present concurrently in PDR.The logistic regression analysis result for correlated danger factors of occurrence of PDR revealed that the relation was inverse correlation between age grade and PDR and positive correlation among DM duration,HblAc,Urine protein excretion rate,hyperlipemia and Yang asthenia scores.The result for DR stages displayed that the risk of a stage aggravation increased 0.068 times with Yang deficiency score added 1 point.The metabonomics outcomes of discrimination showed that DR drew off from normal group completely,Yang and non-Yang deficiency groups were basically separated with few overlap,DR preclinical and non-proliferative phase was overlap and hard to classify,but proliferative could divided from these phases in score plot.There had a certain difference between Yang deficiency and non Yang deficiency groups,and same as proliferative and non- proliferative DR groups.The potential biological markers for Yang deficiency and PDR stage classification belonged to amino acids and organic acids which correlated with human energy metabolism.Conclusion:Both Qi and Yin deficiency was the basic Chinese medical mechanism of DR and throughout the whole duration.Yang deficiency was one of the vital risk factors correlated PDR.,and the essence might be organism energy metabolism diversity,induce the gene expression of new vessels factors by affecting the energy metabolism and internal environment great changes. Yang asthenia was the key pathogenesis of progress influence of DR from the research of the clinical obersavation and metabonolism.