Expression Change Of GABAA Receptor Subunits And Muscarinic Receptor Subunits In Rat Medial Vestibular Nucleus And Expression Change Of Muscarinic Receptor Subunits In Rat Flocculus After Unilateral Labyrinthectomy

PartⅠExpression change of GABAA receptor subunits in rat medial vestibular nucleus following unilateral labyrinthectomyObjective To observe the expression of gamma-aminobutyric acid A receptor (GABAA receptor)α1,β2,γ2 subunits in rat medial vestibular nucleus (MVN) following left unilateral labyrinthectomy (UL). Methods The immunohistochemistry, In situ hybridization histochemistry, Western-blot and RT-PCR were used to observe the expression of GABAA receptorα1,β2,γ2 subunits post-unilateral labyrinthectomy and investigate its effect on vestibular compensation. Results No difference were observe in the ipsilateral and contralateral MVN at any group. No difference were observe in the 1 day, 3 day, 7 day than sham operation MVN following UL. Conclusion Our study suggest that expression change of GABAA receptorα1,β2,γ2 subunits in the MVN most probably were not involved in the early stage of the vestibular compensation process. PartⅡExpression change of Muscarinic receptor subnuits in rat medial vestibular nucleus following unilateral labyrinthectomyObjective To observe the expression of Muscarinic receptor M1,M3,M5 subunits in rat medial vestibular nucleus (MVN) following left unilateral labyrinthectomy (UL). Methods The immunohistochemistry and RT-PCR were used to observe the expression of Muscarinic receptor M1,M3,M5 subunits post-unilateral labyrinthectomy and investigate its effect on vestibular compensation. Results Muscarinic receptor M1,M3,M5 subunits were induced decrease in both side MVN after unilateral labyrinthectomy. The least expression in the 1 day MVN of following UL. The expression is rising from the 3 day to 7 day MVN of following Ul. No difference were observe in the 7 day and sham operation MVN following UL. No difference were observe in the ipsilateral and contralateral MVN at any group. Conclusions Muscarinic receptor M1,M3,M5 subunits were induced decrease in the MVN after unilateral labyrinthectomy. The fall in the resting discharge of the central vestibular neurons may be caused by the decrease of Muscarinic receptor M1,M3,M5 subunits in the MVN. But the significance of the change of Muscarinic receptor M1,M3,M5 subunits in the vestibular compensation is still unknown. PartⅢExpression change of Muscarinic receptor subnuits in rat flocculus following unilateral labyrinthectomyObjective To observe the expression of Muscarinic receptor M1,M3,M5 subunits in rat flocculus following left unilateral labyrinthectomy (UL). Methods The RT-PCR was used to observe the expression of Muscarinic receptor M1,M3,M5 subunits post-unilateral labyrinthectomy and investigate its effect on vestibular compensation. Results Muscarinic receptor M1,M3,M5 subunits were induced decrease in both side flocculus after unilateral labyrinthectomy. The least expression in the 1 day flocculus of following UL. The expression is rising from the 3 day to 7 day flocculus of following Ul. No difference were observe in the 7 day and sham operation flocculus following UL. No difference were observe in the ipsilateral and contralateral flocculus at any group. Conclusions Muscarinic receptor M1,M3,M5 subunits were induced decrease in the flocculus after unilateral labyrinthectomy. The fall in the resting discharge of the central vestibular neurons may be caused by the decrease of Muscarinic receptor M1,M3,M5 subunits in the flocculus. But the significance of the change of Muscarinic receptor M1,M3,M5 subunits in the vestibular compensation is still unknown.