The Presence And Its Clinical Significance Of Circulating DNA In Ovarian Tumors Patients

Objective:Malignant ovarian tumors are the highest mortality of malignant tumors of female reproductive system,and its 5-year survival rate is only about 25%due mainly to compare the clinical incidence of occult,early cancer without any symptoms,the lack of specific indicators of early onset,it more than 70%When found,the others belong to patients with advanced,although in the past 15 to 20 years in the diagnosis and treatment have made a great effort,such as a variety of tumor markers in clinical widely used,surgical techniques continue to improve,as well as platinum,taxol,etc.chemotherapeutic drugs have also been used in clinical,but ovarian cancer patients with 5-year survival rate remained stagnant.Therefore,look for high-ovarian cancer susceptibility genetic markers for ovarian cancer diagnosis and guide clinical treatment and early prevention has important significance.In this study,PCR-SSCP,PCR-RFLP assay in patients with ovarian cancer blood MSPI polymorphism in the CYP1A1 gene,GSTT1 gene polymorphisms and microsatellite instability and susceptibility to ovarian cancer,analysis of CYP1A1 gene MSPI many normality,GSTT1 gene polymorphisms and microsatellite instability in patients with ovarian cancer histological types,such as the relevance of pathological stage.Through the peripheral blood of patients with ovarian cancer circilating DNA for qualitative research to explore a new can be used for early diagnosis and efficacy of the method of monitoring.Method:In this study,first of all,the collection of ovarian cancer patients and control group,blood samples of patients with ovarian cancer clinical data to do a retrospective analysis,using conventional phenol-chloroform extract genomic DNA,the design of specific primers,using PCR-SSCP,PCR-RFLP assay in patients with ovarian cancer blood MSPI polymorphism in the CYP1A1 gene,GSTT1 gene polymorphisms and microsatellite instability and susceptibility to ovarian cancer,for sequencing verified the results with the GenBank sequence database analysis than on statistical treatment,MSPI polymorphism analysis of CYP1A1 gene,GSTT1 gene polymorphisms and microsatellite instability in patients with ovarian cancer histological types,such as the relevance of pathological stage. Through the peripheral blood of patients with ovarian cancer free DNA for qualitative research to explore a new can be used for early diagnosis and efficacy of the method of monitoring.Result:1.In accordance with the FIGO(2000 years) phases:CYP1A1 gene A genotype groupⅠ-Ⅱaccounted for 14 cases of phase 28%,Ⅲ-Ⅳaccounted for 36 cases of phase 72%;B genotype groupsⅠ-Ⅱperiod 14 cases of 48%By statistical analysis at the various genotypes on the clinical stage was no significant difference.CYP1A1 gene A genotype group of 50 patients with epithelial ovarian cancer in 42 cases;B genotype group of 28 cases of patients with epithelial ovarian cancer in 17 cases;C genotype group of 13 patients with epithelial ovarian cancer in 11 cases.Genotype in patients with various pathological types in accordance with epithelial ovarian cancer and non-epithelial malignant tumor group compared with the results of statistical analysis showed that patients with different genotypes than the composition of pathological types were significantly different(x~2=8.22,P＜0.05).2.Ovarian cancer group and control group in the GSTT1 deletion(null) genotype distribution frequency of 38%(35/91) and 12%(6/50),the two groups in the GSTT1 deletion genotype compared there was a significant difference(x~2=10.96,p＜0.05).GSTT1 gene deletion increases the risk of ovarian cancer.Epithelial ovarian cancer and non-epithelial ovarian malignancies in the GSTT1 deletion(null) genotype frequencies were 43.14%(30/70),14%(3/21) found that GSTT1 deletion(null) genotype in epithelial ovarian cancer and compared with the control group there was a significant difference(x~2=9.72, p＜0.05).GSTT1 gene deletion increases the risk of major epithelial ovarian cancer risk.3.Serous ovarian cancer group cystadenocarcinoma,mucinous cystadenocarcinoma,endometrioid carcinoma,Krukenberg tumor and other types happen frequency microsatellite instability was followed by 58.7%(27/46),41.7%(5/12),50%(5/10),0%(0/2),28.6%(6/21). In comparison of the difference was significant(P＜0.05).prompt microsatellite instability and ovarian cancer pathological type. Early(Ⅰ-Ⅱperiod) patients and late(Ⅲ-Ⅳperiod) occurred in patients with microsatellite instability frequencies were 50%(17/34) and 45.6%(26/57).Microsatellite instability in early and late compared in the difference was significant(x~2=0.16,P＜0.05).91 cases of ovarian cancer occur in patients with microsatellite instability frequency of 47.25(43/91),benign and normal human blood was not detected in the microsatellite instability from occurring.Conclusion:1.Ovarian cancer in patients with peripheral venous blood circulation DNA exist microsatellite instability.Microsatellite instability has relationship with pathological types of ovarian cancer,clinical pathological stage.Microsatellite instability exists only with ovarian cancer,does not occur with benign tumors and normal controls.2.Ovarian cancer in patients with peripheral venous blood circulation DNA exist the polymorphism MSPI gene CYPIAI.Malignant epithelial ovarian incidence A genotype(wild-type T/T) higher than that of other genotypes.CYPIAI gene polymorphism and ovarian cancer MSPI has no relationship with pathological stage.3.Ovarian cancer circulating DNA in peripheral blood of patients with genetic susceptibility to the existence of GSTT1.GSTT1 gene deletion in ovarian cancer may be one of the risk factors, GSTT1 gene deletion increases the risk of ovarian cancer,the main increase the risk of epithelial cancer risk.4.The future through the blood of patients with ovarian cancer in the qualitative detection of DNA for the early diagnosis of ovarian cancer,provide a basis for assessment of prognosis.