Single-strand Conformation Polymorphism Analysis On The δ-sarcoglycan Gene In Chinese Patients With Dilated Cardiomyopathy

Postgraduate: Hang Chen Tutor: Professor Li RaoObjective Dilated cardiomyopathy (DCM) is a major cause of morbidity and mortality. Some genes have been identified for the X-linked forms and autosomal dominant DCM. To elucidate the etiology of dilated cardiomyopathy (DCM) in humans, we analyzed the delta-sarcoglycan gene (SG), which is reported to be the causal gene for DCM in the Syrian hamster BIO 14.6. It is also identified as a disease-causing gene responsible for familial and idiophthic DCM in Japanese patients. There is no report about this gene in Chinese DCM patients.Methods We performed polymerase chain reaction (PCR) single-strand conformation polymorphism (SSCP) and nucleotide sequence analyses on the delta-SG in 50 patients with DCM and 50 control cases. SSCP was detected in exon 6 and exon9p of the gene, but not in the other exons.Results In polyacrylamide gel electrophoresis, We have not found abnormal conformer in both affected individuals and control cases. There were no differences in the DNA sequence between these two groups.Conclusion We did not find any amino acid mutation in the 5 -SG gene in patients with DCM, indicating that 6 -SG is not a common causal gene for DCM in the Chinese population.