The Chemical Composition Of Leaves Floral And Small Wax Tree

This dissertation investigated chemical constituents of ethanolic extracts of the root of Adina polycephala and the stem bark of Fraxinus sieboldiana and their bioactivities on several pharmacological models. By using a variety of chromatographic techniques, 50 compounds were isolated, 30 (A1*-A30) from A. polycephala and 20 (F1*-F20) F. sieboldiana. On the basis of spectroscopic and chemical methods, their structures were determined. Their chemical names and structures are listed in Table 1 and Figure 1.Among them, 17 structures are new, including four dimeric phenolic glycosides (A1*-A4*) and a new iridoid diglycoside (5*) from A. polycephala, as well as seven abietane diterpenes (F1*-F7*) and five nor-abietane diterpenes (F8*-F12*) from F. sieboldiana. At a concentration of 10^-5M, A3*, F7*, F10* and F13 showed potent in vitro activities against the release of glucuronidase in rat polymorphonuclear leukocytes induced by platelet-activating factor (PAF), with inhibitory rates of 60.2%, 59.7%, 56.1% and 65.9%, respectively. A13 exhibited nuroprotective activity against serum deprivation-induced PC 12 cell apoptosis with protection ratio of 51%. Compound F14 was active against H5N1 avian influenza virus with IC₅₀ value of 4.8μM. In addition, A4*, F1* and F6* showed selective cytotoxicity against several human cancer cell lines including lung (A549), ovarian (A2780) and lung (A549) cancer cells, with IC₅₀ value of 0.21, 1.72 and 6.04μM.Table 1. Compounds isolated from A. polycephala (A1*-A30) and F. sieboldiana (F1*-F20) "New compounds." The absolute configuration of which was determined.