Study On Biological Compounds In Adina Rubella Hance

This dissertation is composed of three chapters. The first chapter studied the isolation and identification of compounds from Adina rubella. The second chapter studied the a-glucosidase inhibitory, antioxidant, coagulant and hepatoprotective activity of A. rubella. The third chapter summarized the progress of chemical constituent and pharmaceutical activity of Adina Salisb.Chapter1. The chemical constituents of Adina rubellaThe chemical constituents from the A. rubella were studied by column chromatography.14compounds were isolated and identified as a-tocopherol (AR-1), tricosane (AR-5),E-phytol (AR-7), Z-phytol (AR-8), β-sitosterol (AR-9), hexacosane (AR-10), excisanin K (AR-12), oleanolic aicd (AR-13), ursolic acid (AR-14), trans-4’-hydroxy-2’-methoxycinnamaldehyde (AR-15), trans-p-hydroxoy-cinnamic acid (AR-17),5-O-caffeoylquinic acid butyl ester (AR-21),7-epi-vogeloside (AR-22),7-O-β-D-glucosyl-noreugenin (AR-23). Compounds AR-1, AR-5, AR-7, AR-8, AR-10, AR-12, AR-13, AR-14, AR-15, AR-17, AR-21, AR-22were isolated from this genus for the first time.Chapter2. The biological activity of Adina rubellaThe inhibitory activity against a-glucosidase of different extracts from A. rubella was screened in a96-well plate. Petroleum ether extract (IC50=994.0μg·ml-1), EtOAC extract (IC50=862.2μg·ml-1), n-BuOH extract (IC50=994.0μg·ml-1) showed stronger inhibitory effect against a-glucosidase, and were higher than that of acarbose (IC50=1103.01μg·ml-1).DPPH, ABTS and FRAP assays were used to stydy the antioxidant activity of A. rubella. Results showed that petroleum ether extract and n-BuOH extract for the reduction of Fe3+ability were stronger than that of EtOAC extract, EtOAC extract showed higher ABTS and DPPH radical scavenging activity than that of petroleum ether extract and n-BuOH extract.The hepatoprotective activity of A. rubella was investigated using CCl4-induced mice acute liver injury. Petroleum ether extract, EtOAC extract and n-BuOH extract of A. rubella significantly reduced the level of serum GPT, low dose group of the three extracts significantly reduced the level of serum GOT, showed protective effect. Three extracts of A. rubella significantly reduced the level of MDA, and the level of SOD was increased. Results indicated that lipid peroxidation was inhibited by A. rubella to protect CCl4induced mice acute liver injury.Coagulant activity of A. rubella was assayed in vitro. Results showed that petroleum ether extract had procoagulant effect.Chapter3. The study progress of Adina SalisbThe progress of chemical constituent and pharmaceutical of Adina Salisb were summarized.