Studies On The Chemical Constituents And In Vitro Antiviral Activities Of Blumea Laciniata (Roxb.) DC. And Adina Rubella Hance

The chemical constituents and in vitro antiviral activities of Blumea laciniata (Roxb.) DC. and Adina rubella Hance were studied in this thesis.The anti-coxsackie B3 (Cox B3) virus activities, anti-herpes simplex virus 1 (HSV-1) activities and anti-respiratory syncytial virus (RSV) activities of crude extract and different fractions from Blumea laciniata were tested by cytopathic effect reduction assay and MTT assay. As a result, the crude extract, petroleun ether fraction and n-butanol fraction didn't exhibit obvious activities of antivirus. But the ethyl acetate fraction showed anti-HSV-1 activity with the 50% inhibitory concentration (IC₅₀) of 50μg/mL and selective index (SI) of 5, and it exhibited anti-RSV activity with IC₅₀ value of 31.25μg/mL and SI value of 2. The residue fraction exhibited anti-Cox B3 activity with IC₅₀ value of 50μg/mL and SI value of 20, and it exhibited anti-HSV-1 activity with IC₅₀ value of 50μg/mL and SI value of 10.The chemical constitutes of ethyl acetate fraction of Blumea laciniata were isolated. The structures of isolated compounds were elucidated by spectroscopic analysis and comparison with published data. One compound was obtained and characterized to be protocatechuic acid (1), along with a mixture of diosmetin (2) and apigenin (3) and the other mixture of benzoic acid, 4-hydroxy-3, 5-dimethoxy- (4) and scopoletin (5). All of them were found in this plant for the first time. The essential oil of Blumea laciniata was extracted by steam distillation. The chemical constituents in the essential oil were analyzed by GC-MS. Twenty-six components were identified. The contents of the constituents identified make up 86.91% of the total essential oil. The main constituents of this essential oil is (-)-spathulenol (relative content 54.09%). The anti-RSV activities of the essential oil were tested, but it didn't exhibit anti-RSV activities.The anti-RSV activities of crude extract and different fractions from Adina rubella Hance were tested by cytopathic effect reduction assay and MTT assay. As a result, all of the extract and the fractions exhibited anti-RSV virus activities to different extent. The ethyl acetate fraction exhibited the best anti-RSV activity with IC₅₀ value of 15.63μg/mL and the SI value of 16.The chemical constitutes of ethyl acetate fraction and n-butanol fraction of Adina rubella Hance were isolated. Th structures of isolated compounds were elucidated by spectroscopic analysis and comparison with published data. Seven compounds were obtained and characterized to be isovanillic acid (6), aesculetin (7), caffeic acid (8), 5-hydroxy-2-methylchromone-7-O-β-D-xylopyranosyl (1→6)-β-D-glucopyranoside (9), scopoletin (10), 7-O-β-D-glucosyl-noreugenin (11) and scopolin (12). Isovanillic acid (6), aesculetin (7), caffeic acid (8) and scopolin (12) were isolated from this plant for the first time. The antiviral activities of these compounds were tested. Isovanillic acid (6) exhibited strong anti-HSV-1 activity with the IC₅₀ value of 6.25μg/mL, and the SI value of 20, aesculetin (7) exhibited anti-Cox B3 activity with the IC₅₀ value of 25μg/mL, and SI value of 2.5, and anti-RSV activity with IC₅₀ value of 15.63μg/mL, and SI value of 2.