Experimental Research On Interventional Effect Of Supplemented Sini Powder On Changes Of Gastrointestinal Motility In Rats With Liver Cirrhosis

Object:to study how Supplemented Sini powdercan cure rats withliver cirrhosis with gastric motility dysfunction and probe itsmechanism preliminarily by liver function,liver tissuepathology.research of Jiawei Sinisans's prophylaxis the big rats electrogastrograms with liver cirrhosis and conduct a preliminary probe.toprobe into the signal conduction dysfunction of experimental ratscaused by liver cirrhosis with gastric motility dysfunction fromgastrointestinal peptide signal cell level and Supplemented Sinipowdertherapymechanism.touseICC to express c-kit and set it asthe point of entry to study the c-kit change of rats with livercirrhosis and the influence of Jiawei Sinisan,and expecting toexplain partly mechanism how the liver cirrhosis gastric motilitydysfunction happens,using GAS,VIP2receptorsasrepresentatives,to detect the signal receiving system change in gastrointestinalpeptide smooth muscle cells receptor level,and probe into therapycure mechanism of the Jiawei Sinisan.Method:We divide 48 Wister male big rats into 4 groups randomlyand each group has 12 rats,then inject the other 3 groups with40%C C 1₄peanut oil solution with concentration of 2.5ml/kg except blank group,and construct model twice every week and continuouslyfor 11 weeks.After the construction of 4 weeks,using SupplementedSini powderto do gastric perfusion therapy to cure the traditionalChinese medicine group,western group with cisapride gastricperfusion therapy,bank group and model group with physiologicalsaline of the same volume.After 11 weeks,testing all the rats's electrogastrograms,liver function,liver tissue pathology.detect the content changes of MTL,GAS,VIP,SS in serum,gastric,small intestines by radiation immunological.Detect gastrictissue' GAS,SS,c-kit and small intestines' MTL,VIP contentchanges by immunohistochemical technique.detecting the gastricGASR and the jejunum these two receptors' distribution andquantitative analysis by RT-PCR technique.After the test,doingstatistics comparison to the data of traditional Chinese medicinegroup,bank group,modeling group and western medicine group.Results:(1)Rats in normal situation,liver function,liver histolomorphof liver cirrhosis group all had evident differents compared to theblank group(P＜0.01 or P＜0.05).comparison of Supplemented Sinipowdergrouptomodelinggroup:liver function' s TP and ALB contentsincreased obviously,while ALT and AST contents decreased evidentlyand had obvious discrepancies(P＜0.01 or P＜0.05);Liver tissuepathology change alleviated apparently.Comparison of SupplementedSini powdergroup to western medicine group:liver function' s TPand ALB increasedobviously,while ALT,ASTdecreasedevidentlyandhad obvious discrepancies(P＜0.01 or P＜0.05);Liver tissuepathology change alleviated;Supplemented Sini powdergroup' selectrogastrograms was close to normal while western group' s electrogastrograms the amplitude of vibration had slight recover.(2)Modelinggroup' s slow wave rhythm,main frequency,mainpower and fast wave is lower than normal group obviously(P＜0.05orP＜0.01),and have no obvious change of electrogastrogramamplitude of vibration at gastric antrum(P＞0.05);The traditionalChinese medicine group' s slow wave rhythm,main frequency,mainpower and fast wave are bigger than the modeling group(P＜0.05 orP＜0.01),and close to the normal comparison level(P＞0.05),andamplitude of vibration has no evident change than the modeling group(P＞0.05),slow wave rhythm and fast wave are bigger than thewestern medicine group'(P＜0.05orP＜0.01);the'smainfrequencyand fast wave are bigger than the modeling group(P＜0.05),westernmedicine group main power and slow wave rhythm has no evident changethan the modeling group(P＞0.05)(3)To test MTL,GAS,VIP,SS by radiation immunological,theGAS,SS,MTL,VIPcontent inblood,gastric and intestines of livercirrhosis group all had evident increases compared to the bankgroup(P＜0.01orP＜0.05),theGAS,SS,MTL,VIPcontent in blood andgastric of Chinese traditional medicine group all had evidentdecreases compared to the liver cirrhosis group(P＜0.01orP＜0.05),MTL,SS,VIP content in intestines had evident decreases(P＜0.01orP＜0.05),GAS content in intestines had no evidentdecreases(P＞0.05),MTL content in blood had evident increasescompared to the modeling group(P＜0.05),VIPhadevident decreases(P＜0.01),GAS,SS had no evident different(P＞0.05),but haddifferent level of increase or decrease.GAS content in gastric hadevident increases(P＜0.01orP＜0.05),VIP had evident decreases,MTL,SS had no evident different(P＞0.05).GAS in intestines had evident increases(P＜0.05),SShadevidentdecreases(P＜0.05),MTL,VIP hadno evident different(P＞0.05).the GAS,MTL,VIPcontent in bloodand gastric of Chinese traditional medicine group all had evidentdecreases compared to the liver cirrhosis group(P＜0.01orP＜0.05),SShad no evident different(P＞0.05).the GAS,SS,MTL,VIP contentin gastric and intestines of the liver cirrhosis detected byimmunohistochemical technique had obvious increases compared tothe comparison group(P＜0.05),The GAS,SS,MTL,VIP content ingastric and intestines of the Chinese traditional medicine groupall had evident decreases compared to the liver cirrhosis group(P＜0.05),And theMTL,GAS,SS,VIPwas close to the the westernmedicine group,TheGAS,MTL,VIPcontent in gastric and intestinesof the western medicine group all had evident decreases comparedto the liver cirrhosis group(P＜0.05),SS,had no evident different(P＞0.05).(4)In different levels of gastric,c-kit Mean optical density(MOD)and positive area percentage of liver cirrhosis group all hadobvious decreases compared to the comparison group(P＜0.05).Meanoptical density and positive area percentage of the Chinesetraditional medicine group all increased evidently(P＜0.05),andhad no obvious discrepancies to the comparison group.Mean opticaldensity and positive area percentage of the western medicine groupall decreased evidently(P＜0.05),and had no obvious discrepanciesto the modeling group(P＞0.05).(5)All groups had GAS receptor mRNA expression in gastricantrum,and liver cirrhosis group had obvious decreases of GASRmRNAexpression compared to the normal group(P＜0.01);the Chinesetraditional medicine group had evidently increase of GASRmRNA expression compared to the liver cirrhosis group(P＜0.05),hadevidently increase of GASRmRNA expression compared to the westernmedicine group(P＜0.05),and had no obvious discrepancies comparedto the normal group(P＞0.05).All groups hadVIPR2 receptor mRNAexpression in the jejunum,and liver cirrhosis had a higherVIPR2mRNA content than the comparison group(P＜0.01),and theChinese traditional medicine group obviously had a lower VIPR2mRNAcontent than the liver cirrhosis group(P＜0.01).had a lowerVIPR2mRNA content than the western medicine group(P＜0.05).Conclusion:(1)Supplemented Sini powderevident increases of TP and ALBcontents and decreased of ALT and AST content.So,Supplemented Sinipowdercan effectively protect liver cells,recover liver function,and has a good cure of experiment rats' liver cirrhosis andameliorates Gastric motility function(2)Jiawei Sinisans can better experimental rats' electrogastrogram wave dysfunction evidently and promote gastric motility(3)In gastrointestinal peptide signal cell level,GAS,SS,MTL,VIP in blood,gastric and intestines of rats with livercirrhosis had nearly the same change direction,but different inlevel.It maybe relate to liver cirrhosis gastric motilitydysfunction,malabsorption,ascites,etc.Supplemented Sinipowderhad some therapy cure to the experimental liver cirrhosisrats with gastrointestinal peptide parasecretion.(4)Gastric c-kit content in rats liver cirrhosis had evidentdecreased,and Supplemented Sini powdermay have the function ofincreasing c-kit positive ICC.(5)GAS and VIP receptors having different distributions in gastrointestinal tract may have some relationship to the differentgastric motility dysfunction expression caused by liver cirrhosis.Supplemented Sini powderhad a therapy cure to disnormalgastrointestinal peptide receptor level of rats with livercirrhosis.