Experimental Research On Effect Of Shenshuaixiezhuo Pill On The Expression Of Neuclear Factor Kappa B And Osteopontin And Hepatocyte Growth Factor In Rat Kidney With Unilateral Ureteral Obstruction

Objective:To investigate the effect of Shenshuaixiezhuo Pill onNeuclear factor kappa B/p65(NF-κB/p65),Osteopontin(OPN),Hepatocyte growth factor(HGF)of kidney tissue of rats by unilateralureteral obstruction(UUO),and to illustrate the mechanism ofShenshuaixiezhuo Pill on delaying renal interstitial fibrosis.Methods:In the experiment,the rats by unilateral ureteralobstruction(UUO)was adopted.The rats were randomly divided intosham-operated group(n=12),the UUO group(n=12),the low dosageChinese medicine group(n=12),the moderate dosage Chinese medicinegroup(n=12),the high dosage Chinese medicine group(n=12)and thebenazepril group(n=12).It is observed for the change of the commonphysical order,blood urea nitrogen(Bun)and serum creatinine(Scr)ofexperimental rats.HE and Masson staining method for pathologic sliceare used to observe the pathologic change of the experimental rats'kidneytissue after treated and the phase of renal fibroblast.The expression ofNF-κB/p65,OPN,HGF of renal tissue were determined byimmunohistochemistry with two steps.The expression of OPNmRNA ofrenal tissue was determined by RT-PCR.Results:1.Compared to benazepril group,the level of blood ureanitrogen and serum creatinine in the moderate dosage Chinese medicinegroup was markedly intervented(P＜0.05).2.The renal tubulointerstitiallesion degree in moderate dosage Chinese medicine group displayssignifieantly prevention in tubulointerstitial fibrosis,compared to UUOgroup(P＜0.01,p＜0.05).3.The expression of NF-κB/p65,OPN in kidneytissue was decreased,and the expression of HGF was increased in themoderate dosage Chinese medicine group which has significant differencewithbenazeprilgroup(p＜0.01,p＜0.05).4.The expression of OPNmRNAwas also reduced which has significant difference with benazepril group(p＜0.05). Conclusions:1.Shenshuaixiezhuo pill was effective in inhabiting theactivity of NF-κB,reducing the impair resulted from a series ofimmunoinflammation induced by NF-κB,and delaying the development oftubulointerstitial fibrosis.2.Shenshuaixiezhuo Pill can lower OPNexpression level in renal tissue,reduce the impairment oftubulointerstitium.3.Shenshuaixiezhuo Pill can alleviate the renalinterstitial fibrosis in rats with UUO through enhancing the HGFexpression to delay the progression of Chronic kidney disease(CKD).4.Shenshuaixiezhuo pill plays an remarkable role in preventing thedevelopment of tubulointerstitial fibrosis in UUO rats.