| BackgroundEmergency trauma and postoperative wounds in patients with treatment is related to success or failure of surgical treatment,and unexpected events,such as earthquake, fire disaster, military war or remote mountain areas,because of existing multiple factors,like that,the relatively primitive conditions at the scene for medical treatment,complexity of the external environment,long time to transport,so,the significance of the wound protection and treatment is more important. For this reason,most domestic and foreign experts go in for studing prevention of wound infection,the treatment of refractory wound infection,accelerate wound healing for long time. One of the researches related to wound dressings is also one hot spot of a large number of research focus in Burn Surgery and Plastic Surgery.With the development study of wound healing mechanism,people found the purpose of using wound dressing is not only cover wound surface but also refrain bacterium and promote wound surface healing.There have great improvement of biotechnology and industry technology in recent years,and great changes have happened from tradition gauze dressing, biological dressing,synthetic dressing and organize engineering dressing. Although the types have a vast array of beautiful and fine things,all more or less defects must exist,such as traditional gauze which is inexpensive,without the promotion of wound healing and inhibition of wound bacteria;biological dressing,in accordance with its synthesis of raw materials can be divided into a number of species, can speed up the healing,but unable to kill the bacterial and source is limited;organize engineering dressing,is close to the ideal requirements of dressings,because of expensive production costs and higher price,long-term using is unbearable in patients, thereby its promotion and application is limited.This topic studies the development of hollow activated carbon fiber - nanosilver composite dressings manufactured by mutual cooperating of many subjects,in close connection with medical research mainstream direction of the nano-materials in the world,using of nano-materials'specific,like that,the surface effect,small size effect and macroscopic quantum tunneling effect,to be provided with a wide range of medical dressing advantages,including strong adsorption,large tensile strength, good biocompatibility, inhibiting bacterial growth,promoting wound healing, low cost,simple storage conditions. This issue and the results of research can not only serve the clinical, but also in the military war, disasters and poverty-stricken areas,to gain a wide range of applications. Furthermore,there is not study report currently concerned with the hollow activated carbon fiber and nano-silver combinated to service clinical application at the domestic or abroad.Objective1.Preparation of hollow activated carbon fiber - nanosilver composite dressings, observation of the general and microstructur,determination of nanomaterials'unique properties applicable to Medicine.2.Learn the therapeutic effect and safety through the animal experiments of hollow activated carbon fiber - Nanosilver dressing on infected wounds (depth of the deep dermis),including its role in inhibiting bacterial infection in the wound and promote wound healing capacity to provide strong experimental evidence for clinical application in the future.Contents and methods1.Hollow activated carbon fiber-nanosilver composite dressing preparation:carbonize for preparation of activated carbon fiber;liquid Preparation of nanosilver;add al- uminosilicate molecular sieve carrier,complete dressing preparation by integrated spinning.2.Scanning electron microscopy (SEM) observation of the microstructure charac- teristics;application of scanning tunneling microscope (STM),UV-2450 UV Visible Spectrophotometer,x Pert PRO x-ray powder diffraction instrument,FEI Tecnai G 20 TEM and ASAP22400 Automatic physical ad- sorption instrument to determinate of the properties and characterization of materials.3.Animal experiments:set up the wound infection model;designed three test groups,conventional dressing,regularly cut tissue samples,com- parison the efficacy of wound healing, infection control aspects and the security of using hollow activated carbon fiber-nanosilver composite dressing.Results1.General shape of hollow activated carbon fiber-nanosilver composite dressing is similar to traditional gauze,microstructure fibers arranged in an orderly,more uniform length and diameter,aspect ratio to 500:1,aperture 20nm-35nm,length 100μm-150μm,wall thickness 10nm;nano-silver compact structure,particle diameter 5nm.2.Fiber tensile strength than steel with high-volume 100-fold,weight of the latter 1 / 6-1 / 7;silver-containing surface area before and after no significant change;nano-silver ion release of nearly 70%.3.The production of animal models of wound infection from postoperative day 1 every 4 days from determination of a bacterial count, results show that the first 5d, 9d, 13d and 17d of bacteria accumulated inhibition rate, the experimental group than the control group I and II markedly improved. Direct observation by the wound tissue and pathological observations, found that the experimental group than the control group, wound one and two hours after administration of wound healing ahead of schedule 3 ~ 5 days, wound healing time was significantly ahead of schedule. Detection of heavy metals on animal blood had no significant abnormalities, no local and systemic allergic symptoms.ConclusionIt has great applicate scopes because of many features,such as slick,moisten,and can make various kinds shapes according to wound surface's position and size, and have the ability reduce invation of bacterium in wound surface.The inhibiton ration of Nbbcd outweigh other two groups to SA and EC.Nbbcd have the better bacteriostatic action and ability of promote wound surface healing compare with other dressings.All in all,it is the ideal wound dressing in clinical at present.
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