Smilax Anti-inflammatory Substances In The Basis Of Resource Quality Research

Rhizoma smilaci.popularly known in China as "JinGangTeng",belongs to the Liliaceae family Smilax china L.It is commonly used Chinese medicine,which was collected in "the Chinese Pharmacopoeia"in 2005 version.It has various pharmacological effects including relieve sickness wet and disintoxication and so on.Recent pharmacological study has proved it has anti-tumor activity,anti-inflammatory activity and regulation of the immunity activity and so on.The traditional Chinese medicine preparation of "JinGangTeng capsule "and "JinGangTeng syrup",which are made from the main ingredient of Rhizoma smilaci,have good therapeutic efficacy against variouse gynecological inflammation diseases.Under the pharmacological study,this paper is a systematic research carried on the actively anti-inflammatory substances,plant resources and quality of Rhizoma smilaci..Screen the actively anti-inflammatory constituent from the extraction of Rhizoma smilaci.by interdiscipline.The results showed that both ether acetoacetate fraction and n-Butanol fraction have significantly anti-inflammatory activity.The former is the main in gredient of flavones and the latter saponins.14 compounds were extracted and isolated from active fractions by chromatography and spectroscopy.Their structures have been determined as Gallic acid①,Caffeic acid②,Protocatechuicacid③,3,5,4'-trihydroxystibene④,3,5,2',4' -tetrahydroxystilbene(Ⅴ),Kaempferol(Ⅵ),Taxifolin(Ⅶ),Quercetin(Ⅷ),β-sitosterol-D-gluco-side (Ⅸ),Astibin(Ⅹ),Quercetin-4'-O-β-D-glucoside(Ⅺ),Dihydrokaempferol-3-O-a-L-rha(Ⅻ), Quercetin-3-O-a-L-rha(Ⅺ),dioscin(ⅩⅣ).CompoundⅠ,Ⅲ,Ⅶ,X were found in Rhizoma smilaci.for the first time.It is of great significance for Rhizomasmilaci to clarify its materially anti-inflammatory foundation.The mechanism of actively anti-inflammatory substance had been discussed by detecting the content of PGE2 about rat's paws and determining the lever infection of B2(TxB2), F1a(6-keto-PGF1a),IL-1,IL-8,TNF about rat's blood serum by radioimmunoassay.The results showed that the actively anti-inflammatory substances of Rhizoma smilaci.have good inhibition on various inflammatory mechanics.So,it provides scientific evidence of clinical effects about the traditional Chinese medicine preparation made from the main ingredient of Rhizoma smilaci..Based on the research of its materially anti-inflammatory foundation,we extracted and purified anti-inflammatory avtivity fraction,which is Chinese herb standard extract,by Chinese traditional medicine modem general character essential technology.The contents of flavones and saponins in standard extract of Rhizoma smilaci.were researched by UV. The content of Quercetin was researched by HPLC,too.Then,the Fingerprints of the standard extract were established by HPLC-UV and HPLC-ELSD.All of above researches are responsible approach,which correspond with the technical requirements of the Chinese Pharmacopoeia(2005)and the State Food and Drug Administration.So,it builded up the quality assessment system geared to the international standards.At the same time,it laid on the hard foundation for the new creation of traditional Chinese medicine preparation made from the main ingredient of Rhizoma smilaci..In order to grasp the information about hizoma smilaci.'s distributed characteristic, reserves and ecological environment.we have investigated the resources of Rhizoma smilaci. in more than 10 provinces such as Hubei,Hunan,Jiangxi,An'hui,Sichuan,Guangdong, Guangxi,Fujian,Zhejiang,Chongqing;studed it's ecological environment information about terrain,altitude.the nature of soil;analyzed it's distributed characteristic;also compared to analysis of content in Rhizoma smilaci.from different areas.All expected objectives have been obtained.Thus,it is of great significance to utilize properly resources of Rhizoma smilaci.and control the quality of products made from the main ingredient of Rhizoma smilaci..Meanwhile,the paper provides scientific evidence of perfecting GAP and lays on the hard foundation for the sustainable development and utilization of Rhizoma smilaci..