Its Anti-inflammatory Activity Component Triterpenoids Burnet

Radix Sanguisorbae is the dried root of Sanguisorba officinalis L.or S. officinalis L. var. longifolia (Bertol.) Yu et Li (Rosaceae). It’s widely distributed in the northern districts of China. It’s cold in nature, bitter and sour in taste. Its root has hemostatic, analgesic and astringent properties, and it has the efficiency of subsidence of swelling and evacuation of pus, removing toxic materials and treatment of sore. It has been used as a herbal drug for the treatment of bums, scalds, internal hemorrhage, enteritis and dermatogic disease.Previous investigations have shown that the roots of S. officinalis mainly contain tannins, phenolic acids, triterpenoids, flavonoids, anthraquinones, eyanophoric glycosides and sterols. It has also been reported that this crude drug has exhibited many pharmacological activities, including haemostasis, anti-inflammatory, antibiosis, anti-tumor, and promoting wound healing.In this study, chemical constituents of the roots of S. officinalis from Jilin province and its anti-inflammatory activity were investigated.70% ethanol extracts were chromatographed on macroreticular resin column and silica gel column, combined with Sephadex LH-20 and ODS column chromatography to give 10 compounds. Seven compounds’ structures were elucidated by physico-chemical properties and spectral analysis. They were identified as ziyu glycosideⅠ(1)、niga-ichigoside (2), 3β-[(α-L-arabinopyran osyl)oxy]-29-hydroxyolean-12-en-28-oic acid-β-D-glucopyranosyl ester (4)、ziyu glycosideⅡ(7)、ursolic acid (8)、3β-[(α-L-arabinopyranosyl)oxy]-urs- 12,18-dien-28-oie acid fl-D-glucopyranosyl ester (9)、daucosterol (10). Compound 2 and 4 were isolated from this genus for the first time.A review on the triterpenoid saponins, spectral features and pharmacological activities of the genus sanguisarbae was provided, based on some references and auther’s opinion.Furthermore, the anti-inflammatory activity of the saponins 1、2、7 were evaluated using the xylene-indueed mice ear edema, which displayed 15.70%, 31.73% and 29.70% inhibition seperately.