Studying On The Regulating Effect And Mechanism Of Electroacupuncture At Zusanli In Rats With FGID Based On The Theory Of Acupoint And Visceral Coupling

Objective:To explore the special interation between Zusanli and stomach through observing the regulating effect and mechanism of electroacupuncture(EA) at Zusanli in normal and model rats with functional gastrointestinal disorders(FGID).Methods:80 SD rats were randomly divided into normal and model,included 10 groups with 6 cases in each according to the difference of Zusanli and Sanyinjiao.FGID model was established by intraperitoneal injection of reserpine.EA was carried on Zusanli and Sanyinjiao.The microcirculatory blood flow was observed with a laser doppler blood flow monitor in the surface of stomach,duodenum,liver and brain,and the bioelectrical feature of stomach and intestine detected with multi track polygraph.The neurotransmitter and gut hormone in the blood and tissues such as 5-HT,DA,NE,E,MTL,GAS and ET were detected with the methods of radioimmunity and high performance liquid chromatography.Methods of immunohistochemistry and in situ hybridization were used to detect the expression of 5-HT,VIP and VIPR₂ mRNA.Results:For the normal rats,the microcirculatory of stomach and intestine was improved by EA,especially Zusanli 2 group(P＜0.05).The amplitude was decreased,while the frequency of bioelectricity was increased,Zusanli is more significant than Sanyinjiao at the frequency(P＜0.05).The content of ET was reduced by EA(P＜0.05),the content of MTL,GAS was enhanced,the content of NE,E and DA was raised by EA,while 5-HT was not significant.The expression of 5-HT,VIP,VIPR₂ mRNA in normal stomach was enhanced in stomach,intestine and brain(P＜0.05).For the model rats,the descendent microcirculatory of stomach and intestine was improved by EA,and Zusanli was more apparente than Sanyinjiao in the microcirculatory of stomach.The amplitude was decreased,while the frequency of bioelectricity was increased,The ascendant content of ET was reduced(P＜0.05),the content of MTL was enhanced,the serum and intestine content of GAS was increased,the stomach's was reduced,the content of E,DA,5-HT in hypothalamus was raised,the serum content of 5-HT was reduced by EA.The ascendent expression of 5-HT was depressed,and the decendant expression of VIP,VIPR₂ mRNA was enhanced in stomach,intestine and brain of the model rats by EA(P＜0.05).Conclusion:Microcirculation,bioelectricity and related biochemical indicators of the normal and model rats with FGID can be effectively regulated by EA at Zusanli.That may be verified that Zusanli is a certain specific point to stomach and has special relations between them,which provided scientific evidence for the theory of acupoint and visceral coupling.