Model Establishment Of Gastric Ulcer Of Due To Ligating Pylorus Combined With Cold And Hot Syndrome And Study Of Influence On Associativity Prescription On The Model

Objective of the researchFound pyloric ligature model of cold and pyretic syndrome ulcer whichhas the fortes of easy operability,good constancy and repeatabilty,drawassistance from the model to reveal the essence and material foundation ofGastric ulcer of cold and pyretic syndrome though the observation on rules ofcold and pyretic syndrome of Gastric ulcer,research function mechanisms andmaterial foundation of cold or heat formulas(Dahuang Huanglian XiexinDecoction,Lizhong Pill) and rules of recipe corresponding of syndrome,offer the rationale basis and experimental evidence for pathogenesis obtainedthrough differentiation of symptoms and signs in the traditional Chinesemedicine clinic and provide the study on traditional Chinese medicinesyndromes in the mode of combination of diseases with syndrome with thereference in the technology.It has an important sense for the diagnose,pathogenesis obtained through differentiation of symptoms and signs,treatment and study on normalization of pathogenesis obtained throughdifferentiation of symptoms and signs in the traditional Chinese medicineclinic.Method and content of the researchBased on academic thought of professor Li Ji's recipe corresponding tosyndrome and results of the peptic ulcer and literature research from his team,regard pathogenesis of cold or pyretic syndrome obtained throughdifferentiation of symptoms and signs in traditional Chinese medicine as theprinciple.Found pyloric ligature model of cold and pyretic syndrome ulcerbased on the traditional chines medicine theory of etiological factor andpathogenesis and modern medicine methods by means of authenticatingcontradistinction of recipe corresponding to syndrome and means ofduplicating pyloric ligature Gastric ulcer model of rats and exerting cold andheat factors(mixed liquor of ice water +0.3 mol/ml NaOH or 8% dry paprica+60% alcohol) on the model rats.Observe the effect of cold formula Dahuang Huanglian Xiexin Decoction and heat formula Lizhong Pill on the foundedmodel at the recipe corresponding to syndrome.Killed the rats seven hoursafter water-stress.Detected the ulcer index,the content of TNF-a,IL-8,ET,NO and INOS in blood serum.Detected the EGF,TGF-a,SOD andMDAin the stomach tissue.Detected the content of 5-HT and NE in the brain tissue.Results(1) In the pyloric ligature model of pyretic syndrome,SOD,EGF andTGF decrease,MDA,NO,iNOS,IL-8 and INF-αexpression,Gas and NE stepup.Dahuang Huanglian Xiexin Decoction can raise SOD,decrease MDA,irritate the Gastric mucosa to release EGF and TGF-α,restrain IL-8,TNF-αand GAS and decrease sympathesis-adrenal medulla function to make thecontent of NE down.Its function mechanism against Gastric ulcer may beconcerned with releasing the damage to Gastric mucosa by means ofrestraining the lipid peroxidation from free radical in the body,inflammationreaction and Gas secretion,with enhancing the defensive capability in theGastric mucosa and with adjusting the function of central nervous system.(2) In the pyloric ligature model of cold syndrome,SOD,NO,TNOS,NO/ET,EGF and TGF decrease,MDA,ET,TNF-αexpression,Gas and 5-HTstep up.Lizhong Pill can raise SOD and NO,decrease MDA and NT,adjustET/NO,irritate the Gastric mucosa to release EGF and TGF-α,elevatesympathesis-adrenal medulla function to make the content of 5-HT down andrestrain Gastrin.Its function mechanism against Gastric ulcer may beconcerned with releasing the damage to Gastric mucosa by means ofrestraining the lipid peroxidation from free radical and GAS secretion,withenhancing the defensive capability and blood current in the Gastric mucosaand with adjusting the function of central nervous system.Conclusion(1) In the pathology cold and heat formulas can ameliorate or preventand cure the changes of pyloric ligature animal model of cold and pyreticsyndrome Gastric ulcer which is founded by means of combining pyloricligature with cold and heat factors.This is an experimental result of recipe corresponding to syndrome and indicates that this method to found Gastriculcer of cold and heat syndrome is successful.(2) There are differents between heat syndrome and cold syndrome ofpyloric ligature Gastric ulcer in the diversity of indexes including semeiology,anti-oxidation capability,blood current in the Gastric mucosa,inflammatory factor,neurotransmitter,gut hormone and growth factor,revealthe essence micro-indications.(3) Dahuang Huanglian Xiexin Decoction,Lizhong Pill can aim directlyat the pyloric ligature Gastric ulcer of cold or heat syndrome.That is a resultof Recipe Corresponding to Syndrome.This research can offer the reference in technology for the study on theessence of combining disease with syndrome and establish the foundation oftheory and experiment for the further development of traditional Chinesemedicine.