Study On Decocting Method Of Dahuang Huanglian Xiexin Decoction Of Impregnated Xuyu In Mafei Soup

Objective:Throughout the ages,Dahuang Huanglian Xiexin Decoction method controversial lot,To this end,this subject through the experimental method to explore the Dahuang Huanglian Xiexin Decoction "Ma Fei Tang" specific temperature and "XuYu" of the time range.Methods:The model of rat Biochemical Indicator Of spepsin activity?ulcer index?Thromboxane(ET-1)?Superoxide dismutase(SOD)?Malondialdehyde(MDA)?epidermal growth factor(EGF)?transforming growth factor(TGF?-1).prostaglandin(PGE2)ahd based on the leaching of emodin,berberine and baicalin in Dahuang Huanglian Xiexin Decoction.The activity of pepsin was determined by the method of maize.The ulcer index was calculated by Guth standard.The levels of nitric oxide,thromboxane(ET-1),superoxide dismutase(SOD)and propoxidase(SOD)were measured by enzyme-linked immunosorbent assay(MDA),epidermal growth factor(EGF),transforming growth factor(TGF?-1),prostaglandin E2(PGE2)and high performance liquid chromatography(HPLC)were used to determine the content of emodin,baicalin,Berberine three components content.Results:The ulcer indexes of A,B,C,D,E,F,G and H were significantly decreased(P<0.01 or P<0.05),and there was significant difference between the two groups(P<0.01 or P<0.05),In group F,the ulcer index was the lowest and the ulcer coefficient was 0.33 ± 0.58,and the effect of ulcer repair was the best when the Dahuang Huanglianxixin Decoction was immersed at 85? for 48min;A,C,E,G group compared with the model group,the four groups of pepsin activity has a very significant statistical significance(P<0.01),As a benchmark,Dahuang Huanglianxixin Decoction was best at 00? for 48min;There was no statistically significant difference between the two groups(P<0.05).There was significant difference between the model group and the blank group(P<0.05);The levels of NO in group A,B,C,D,E,F,G and H were significantly different from those in model group(P<0.01),and there was significant difference between group I and model group(P<0.05)ET-1 content was significantly different from that of model group(P<0.05).Based on these two indicators can be obtained at 85? for 15 minutes immersion effect is better;The SOD content in group D was significantly different from that in model group(P<0.01),E group compared with the model group was statistically significant(P<0.05),MDA content of E,F,I group and the model group was significantly statistically significant(P<0.05)Compared with the model group,There was no significant difference between the other groups and the model group(P<0.05),No statistically significant(P<0.05).The combination of two indicators can be obtained at 85? for 15 minutes,the best effect;From the PGE2 content,E group compared with the model group was statistically significant(P<0.05),There was no significant difference between the other groups and the model group(P<0.05);The expression of TGF?-1 in E and G groups was significantly different from that in model group(P<0.01),There was significant difference between F group and model group(P<0.05),There was no statistically significant difference between the other groups and the model group(P<0.05).There was significant difference between D group and model group(P<0.05),There was no significant difference between the other groups and the model group(P<0.05).The optimal dipping conditions for these three biochemical indicators were impregnated at 85 ? for 15 minutes.The concentration of emodin was the highest at 70 ? for 15 min,and the concentration of berberine was the highest at 110? hot water for 48 min.The concentration of baicalin was the highest at 85 ? for 48 min.Conclusion Through the animal experiment and high performance liquid chromatography,we can get the best impregnation condition of Dahuang Huanglian Xiexin Decoction for 85min under the condition of 85 ?.