| ObjectiveDahuanghuanglianxiexin Tang and Lizhong Wan(Tang) were classicalprescriptions in healing digestive system diseases, the former was used totreating heat sydrom, but the latter was opposite. Based on literature, clinicaland experimental research about the two prescriptions, we studied thephamacological effect and possible mechanisms of the two prescriptions inhealing acetic acid induced gastric ulcer in rats. The study aimed to determinethe different mechanisms on treatment to the same syndrome-gastric ulcercaused by acetic acid of the two prescriotions, which can treat syndromes ofdiametrically opposite properties of cold and heat.Methods1)50 Wistar rats were randomly divided into control group, sham-operatedgroup, model group, Dahuanghuanglianxiexin Tang group and LizhongWan(Tang) group. Gastric ulcer was induced by the glacial acetic acid method.Dahuanghuanglianxiexin Tang group and Lizhong Wan(Tang) group wereadministrated by gastric perfusion of the same volume ofDahuanghuanglianxiexin Tang and Lizhong Wan(Tang) once every day, andthe other three groups were given the same volume of normal saline onceevery day. All the groups were observed continuely 14 days.2) The gross stucture of rat stomach was observed, the longest vertical andhorizontal diameters were measured by the vemier caliper and then calculatedthe ulcer area according to the fomula.3) The rat blood was collected and centrifuged, and the obtained supematantwas serum. The content of NO, iNOS, ET, IL-8, TNF-αwas tested.4) The stamoch tissue were taken and homogenated, the activity of SOD, thecontent of MDA, EGF, TGF-αwas tested.Results1)Effect of the two prescriptions therepy on the healing of gastric ulcerThe two prescriptions can shrink the size of ulcer area. There was asignificant difference between model group and the two treatment groups(P＜0.01).2)Mechanism of Dahuanghuanglianxiexin TangThe activity of SOD had been improved, which was significant differentbetween treatment group and model group(P＜0.01). But there was no obviouseffect on MDA.The content of ET had been decreased, which was significantly differentfrommodel group, (P＜0.01), but the content of NO, iNOS had no obviouschanges.The content of IL-8 had been decreased, which was significantly differentfrom model group, (P＜0.01). But there was no obvious effect on TNF-α.The content of GAS had been decreased, which had significant difference withmodel group, (P＜0.05).The content of EGF and TGF-αhad been increased, which were significantlydifferent from model group(P＜0.01).3) Mechanism of Lizhong Wan(Tang)The activity of SOD and the content of MDA had been both increased, andthere were significant difference between treatment group and modelgroup(P＜0.01).The content of Nohad been increased and the content of ET had beendecreased, and there were significant difference between treatment group andmodel group(P＜0.01).The content of IL-8 had been decreased, and there was obvious differencewith model group, (P＜0.05). But there was no obvious effect on TNF-α.The content of EGF and TGF-αhad been increased, which were significantlydifferent from model group(P＜0.01).Conclusions1) The two prescriptions had good curative effect on gastric ulcer in rats.2) The possible mechanism of Dahuanghuanglianxiexin Tang was that theprescription can reduce the content of GAS and ET, increase the content ofEGF, TGF-α, inhibit the release of IL-8 and improve the activity of SOD.3) The possible mechanism of Lizhong Wan(Tang) was that the prescriptioncan increase the content of NO, decrease the content of ET, and then revertNO/ET to the balance condition, inhibit the release of IL-8, improve theactivity of SOD, and at the same time reduce the content of MDA, increase the content of EGF, TGF-α.3) Dahuanghuanglianxiexin Tang treated heat syndrome of gastric ulcer, whileLizhong Wan(Tang) treated cold syndrome of gastric ulcer. Although they hadsame therepeutic effects on the same gastric ulcer in model rats, themechanisms of those two prescriptions were different.The results proved that it was proper and necessary of sydrome differentiation,and treatment correspondence between prescriptions and sydromes. And theresults also provided the experimental evidences and data for the clinicalapplication of the two classical prescriptions.
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