The Comparative Study On Gene Expression Of Kidney-YIN Deficiency And Kidney-YANG Deficiency

[ Background]The syndrome of Traditional Chinese Medicine is the summarization of etiopathogenisis in different stage of diseases. Since same symptome have common clinical manifestation and pathomechanism, then they have common material foundation, but this material probably concerned with gene or genome. The essence in kidney which come from essential substance for reproduction of the parents, is the hyle of embryonic development, it identity with DNA in modern medicine. Therefor, it is significant to study the molecular mechanism of nephrasthenia syndrome from the aspect of genome, in elucidation substance of nephrasthenia syndrome. As both of the basic syndrome, Kidney-yin deficiency and Kidney-yang deficiency is, family history of Kidney-yin deficiency and Kidney-yang deficiency medical certification and control of the theory are quite seriously. Syndrome of Kidney-yin deficiency and syndrome of Kidney-yang deficiency reflects functions of the overall body immediately, this basic contradiction is the "disease" of the interference. The difference between syndrome of Kidney-yin deficiency and syndrome of Kidney-yang deficiency in different diseases is because of the specificity of diseases. Only to study the difference and to induce general rule of syndrome, then we have all round knowledge of syndrome of Kidney-yin deficiency and syndrome of Kidney-yang deficiency. Accordingly, it is necessary to combine disease with syndrome to research syndrome of Kidney-yin deficiency and syndrome of Kidney-yang deficiency of differential gene expression.cDNA Lib of Kidney-yin deficiency (including diabetes, chronic nephritis, lupus nephropathia and sub-health)was successfully set up in our prior study works by some techniques and methods, such as RNA microamount-amplification, differential hybridization and gene cloning. The results showed that Kidney-yin deficiency of the different diseases in the subtractive library card there is an obvious difference, and Kidney-yin deficiency sub-health subtractive library card is very similar to the common library. This prompted the construction of subtractive cDNA library of Kidney-yin deficiency and evidence of the disease must be interference. That is, differences in selected parts of the gene may be associated with the disease and Kidney-yin deficiency of the card has nothing to do with the interference of the disease, the most minimum disruption is Kidney-yin deficiency of sub-health in subtractive library card. This may be due to sub-health between health and disease is intermediate, although the disease does not meet, but has shown Chinese medicine syndromes are functional changes, it is most pure syndromes in the nature. This indicates that it is very important significance of the sub-health for the essence of TCM syndrome research.The technology of gene chip is a revolutionary milestone of bioinformatics analysis field, It has a high-throughput, miniaturization and automation features, has become a highly efficient, rapid and large-scale access to information related to an important means of biological. As an emerging bio-technology, gene chip is widespread concern around the world. At present, The technology of gene chip has been widely used in many areas of TCM research. How the disease to Western medicine and the syndrome of traditional Chinese medicine are unified together? Gene expression may be an important link point. Therefore, gene expression offered unprecedented opportunity for research the essence of TCM Syndrome.With the method of disease combined with syndrome and the technology of gene chip, to complete the comparative study of gene expression of Kidney-yin deficiency and Kidney-yang deficiency. Accordingly, the objective of the study is to find differential expression gene of Kidney-yin deficiency and Kidney-yang deficiency and to approach the mechanism of Kidney-yin deficiency and Kidney-yang deficiency in the level of gene.[Methods]1,Using "disease combined with syndrome", the patients with Kidney-yin deficiency and Kidney-yang deficiency of sub-health or IgA nephropathy were divided into experimental group. Those healthy people were divided into normal control group.2,Collected cases and preparation samples, used of gene chip technology for chip hybridization and scanning analysis. Study on gene expression profiles of Kidney-yin deficiency and Kidney-yang deficiency. According to the results of gene chip analysis, randomly selected differential expressed genes of Kidney-yin deficiency and Kidney-yang deficiency for RT-PCR validation.3,Combination of MAS software, analysis differential gene expression tosearch the internet using public biological databases by http://www.genesifter. net/web/index.html and http://www.geneontology.org. Pathway is analysised by KEGG(kyotoencyclopedia of genes and genome, http://www.genome.ad.jp ) and BioCarta(http://www.bioCarta.com) and GenMAPP (http://www.genmapp.org) . [Results]1,There is characteristic profiles of differential expressed genes between Kidney-yin deficiency group and normal control group. Among a total of 79 differential expressed genes. Increase the expression level (Ratio values greater than 2) has six, Reduced expression level (Ratio value of less than 0.5) there is the 73 .These differentially expressed genes in their analysis according to the functions of the general classification. Mainly genes related to immune function, metabolism, protein biosynthesis, oxidative stress, the process of genetic information, ion channels, protein amino acid phosphorylation go, apoptosis, cell signal transduction. These differentially expressed genes involves a number of signal transduction pathway, such as, Axon guidance pathway,Phosphoprotein phosphatase activity pathway,Cytoplasmic vesicle pathway,G Protein Signaling pathway,Calcium signaling pathway,MAPK signaling pathway, etc.2,There is characteristic profiles of differential expressed genes between Kidney-yang deficiency group and normal control group. Among a total of 75 differential expressed genes. Increase the expression level (Ratio values greater than 2) has 22, Reduced expression level (Ratio value of less than 0.5) there is the53 . Mainly genes related to immune response, metabolic pathways, signal transduction pathways, Cytokines, apoptosis, cell receptors, and cytoskeleton, oxidative stress, Ion channels (calcium, magnesium, zinc, copper), DNA binding, transcription factor, protein synthesis Translate . These differentially expressed genes involves a number of signal transduction pathway, such as, Adherens junction Pathway,Cytokine-cytokine receptor interaction pathway,MAPK signaling pathway (mitogen - activated protein kinase),Wnt signaling pathway,Calcium signaling pathway,Receptor signaling protein activity,Trypsin activity pathway,Magnesium ion binding pathway,TGF-beta signaling pathway etc. 3,There was a clear difference of gene expression profiles of Kidney-yin deficiency and Kidney-yang deficiency through a comparative analysis. All of the differential expressed genes has 145, detailed analysis see the above paragraph of article 1,2. Among of Kidney-yin deficiency group,Kidney-yang deficiency group and normal control group, the common differential expressed genes has 9. Including CA8,SYPL1,PHF14,ARHGAP18,PYROXD1,RECQL,OSTF1,SEC23B,231418_at etc. These genes related to immune function, metabolism, Cell cycle, bone development, DNA repair,protein biosynthesis, etc. 231418_at gene function is not clear.[Conclusions]1,Gene-chip is a more ideal method of studying TCM Syndrome associated genes.2,Using "disease combined with syndrome", carryed out the comparative research on differential gene expression of Kidney- yin deficiency and Kidney- yang deficiency. There is a initial evidence of Kidney-yin deficiency-related genes, a total of 79. There is a initial evidence of Kidney-yang deficiency-related genes, a total of 75.3,There is differential gene expression between Kidney-yin deficiency and Kidney-yang deficiency, tips for TCM Syndrome types of Kidney-yin deficiency and Kidney-yang deficiency with evidence and scientific basis. Compared with normal control group, there is differential gene expression in common of Kidney-yin deficiency and Kidney-yang deficiency, show that there is performance of Kidney deficiency between Kidney-yin deficiency and kidney-yang deficiency, To provide a scientific ideas and methods for reveal the essence of TCM. It was the foundation on to explore the Kidney-yin deficiency and Kidney-yang deficiency mechanism in the gene level and further development of Kidney-yin deficiency and Kidney-yang deficiency of the gene chip.