Observation The Clinic Effect Of Qianlongtong Capsule On Treatment Of Benign Prostatic Hyperplasia And The Influence On Prostatic Matrix Cell TGF-β₁ MRNA Express

Purpose:1.Observe the clinical curative effect of Qianlongtong capsules treatment BPH.2.The influence of Qianlongtong capsules for body outside training person BPH prostatic stroma cells TGF-β1 mRNA expresses.Test partMethods:1.Cell culture method.2.MTT detects the influence to stroma cell increasing of different thick degree Qianlongtong 3.RT-PCR expand -increasing law,observe the influence of different density Qianlongtong capsules TGF in training matrix cell-β₁ mRNA expresses; At the same time apply MTT to detect the influence of stroma cell for training hyperplasia.Results:Different density Qianlongtong capsules(medicine liquid) role after,train TGF-β1 mRNA express the reduction that arises different level,high dosage Qianlongtong capsule(medicine liquid) TGF-β₁ mRNA express minimum(0.61±0.32).High density group is compared with blank group(P＜0.01),high dosage is organized with medium dosage group is compared with low thick degree group TGF-β₁ mRNA express weaken obviously(P＜0.05,P＜0.01),but low dosage group is compared with blank group do not have obvious discrepancy(P＞0.05), cell blank-controlled group TGF-β₁ mRNA express highest.In training cells,different dosage Qianlongtong capsule not at the same time intercropping use after,for prostate matrix cell hyperplasia to show make inhibition,and show dosage effect stand,high and medium thick degree groups are obvious inhibition,in the OD value of thick group in 24 h,48 h,72 h and 96 h for prostate cell growth have,with cell blank compare group comparison(p＜0.01),and with medicine density submit dosage dependence;It is compared that the OD value of low density group has inhibition and cell blank from a little beginning of 72 h for prostate cell growth to compare group(p＜0.05),in 24 h and a little and cell blank of 48 h compare group compare without obvious discrepancy(p＞0.05).Conclusion:Qianlongtong capsule in test tube can restrain prostatic stroma cell hyperplasia,can reduce TGFβ₁ in matrix cell in express.Clinical partialMethod:For 60 examples,case has carried out clinical observation.60 cases of BPH were divide into treatment group and controlled group randomly,30 cases in treatment group taking orally Qianlongtong capsules,30 cases in controlled group take orally Lngbishu capsules, observe patient urination symptom give a mark,live quality give a mark, and adopt B to exceed bladder remaining urine and the prostate volume of detection BPH patient quantity,flow out idea with urine to measure biggest urine flow out rate and average urine flow out rate.As a result:Qianlongtong capsules raising the biggest urine of patient flow out rate and decreasing bladder remaining urine the aspects such as quantity is better than obviously Longbishu,clinical curative effect has significance discrepancy(p＜0.01),can reduce the prostate volume of hyperplasia,with controlled group comparison(p＜0.05).Conclusion:Qianlongtong capsule raising the biggest urine of patient flow out rate and decreasing bladder remaining urine quantity is better than obviously Longbishu;Can reduce the prostate volume of hyperplasia.Pointing out Qianlongtong capsule treatment prostate hyperplasia disease possess wide application prospect.