The Experiment Study Of Myocardial Angiogenesis Of Heart Blood Stasis Syndrome And The Effect Of Treated By Yang Xin Tong Mai Effective Component Recipe

Partâ… theoretical researchObjective:To research the relevance between the theory of collateral disease and angiogenesis, probe the basic pathological basis of HBSS from perspective of theory of collateral disease.Methods:using literature research,documents from the CNKI database and VIP database which relevant with theory of collateral disease and angiogenesis.Conclusion:1,Through research that when theory of collateral disease applied to coronary heart disease, there has highly relevance with angiogenesis.Mainly reflected in the theory of collateral disease have the inference relevance with blood vessels and nerves,neural-endocrine-immune network and vascular endothelial injury in modern medicine.2,The basic theory of collateral disease of HSBB is xinluobizu,xinluochuji and luoxuburong.Partâ…¡Research PrescriptionExperiment 1:Experimental study of Yang Xin Tong Mai Recipe effective component parts anti-Acute myocardial ischemiaObjective:to determinate Yang Xin Tong Mai Recipe main effective componentMethods:Randomly divided Wistar rat into 10 groups Blank model group,normal comparison group,total alkaloid group,compound prescription glycosides group,compound prescription aglycone group, the compound prescription protein group,the salvia miltiorrhiza phenanthrenequinone group, the ginseng polysaccharide group,the compound prescription polysaccharide group and the total volatile oil group.All groups except normal comparison group were established with Pit inject via caudal vein.Every rat was examined15s,30s,2min,5min,10min electrocardiogram ECG after they were in narcosis,then take Carotid artery blood to examine Whole blood viscosity and Plasma viscosity.Result:(1) Electrocardiogram change degree:â‘ The Ginsenoside,tanshinoneâ…¡A,The ginseng polysaccharide,the compound prescription polysaccharide,the total alkaloid,the total volatile oil,the salvia miltiorrhiza phenanthrenequinone 7 groups' J point displacement have Significant statistical significance, Compared with the model group(P＜0.05 or P＜0.01).â‘¡The compound prescription protein,the compound prescription aglycone and the compound prescription glycosides groups' J point displacement have non Significant statistical significance Compared with the model groups(P＞0.05);(2) Whole blood viscosity and Plasma viscosity change: â‘ The Ginsenoside,the tanshinoneâ…¡A,the total alkaloid,the ginseng polysaccharide,the total volatile oil can obviously improve the rat's blood rheology indicators,compares with the model group has the extremely remarkable statistics difference(P＜0.01);â‘¡Compound prescription aglycone,compound prescription glycosides then the obviously increase blood hemorheology indicators(Whole blood shear rate),compares compound prescription aglycone group with the model group to have the remarkable statistics difference (P＜0.01).â‘¢Compound prescription glycosides group,compound prescription aglycone and the model group no significant difference in the Plasma viscosity(P＞0.05);Conclusion:The Ginsenoside,tanshinoneâ…¡A,ginseng polysaccharide,the compound prescription polysaccharide,the total alkaloid,the total volatile oil,the salvia miltiorrhiza phenan threnequinone 7 component parts have the anti-acute myocardial ischemia initiated by Pit,however the compound prescription protein,the compound prescription aglycone,the compound prescription glycosides does not have the anti-acute myocardial ischemia function. So,we take The Ginsenoside,tanshinoneâ…¡A,ginseng polysaccharide,the compound prescription polysaccharide,the total alkaloid,the total volatile oil 5 components as the main effective components and exclude the compound prescription protein,the compound prescription aglycone,the compound prescription glycosides 3 No Role component parts.Experiment 2:Experimental study of Yang Xin Tong Mai Recipe effective component and the Secondary effective components parts orthogonal interaction on anti-Acute myocardial ischemia.Objective:to clear if there have interaction between five major components parts and two minor components partsMethods:take The Ginsenoside,tanshinoneâ…¡A,ginseng polysaccharide,the total alkaloid, the total volatile oil as the basic factor,the compound prescription polysaccharide,the salvia miltiorrhiza phenan threnequinone as Investigation factors.According L4(2³) orthogonal design and arrange the experiments.Randomly divided 40 Wistar rats into 5 groups:Blank model group,1 prescription group,2 prescription group,3 prescription and 4 prescription group.All groups were established with Pit inject via caudal vein.Every rat was examined15s,30s,2min,5min, 10min electrocardiogram ECG after they were in narcosis,then take Carotid artery blood to examine whole blood viscosity and plasma viscosity.Result:(1) Electrocardiogram change degree:â‘ Here have significant statistical significance 1 prescription group,2 prescription group,3 prescription and 4 prescription group compared with the model groups in 15s,30s,120s,300s, 600s after module established(P＜0.05).â‘¡differences of level 1,level 2 were not statistically significant(P＞0.05).(2) Whole blood viscosity and Plasma viscosity change:â‘ Here have significant statistical significance 1 prescription group,2 prescription group,3 prescription and 4 prescription group Compared with the model groups(P＜0.05). â‘¡differences of level 1,level 2 were not statistically significant(P＞0.05).Conclusion:there have no interaction between five major components parts and two minor components parts.Experiment 3:Experimental study of Yang Xin Tong Mai Recipe effective component effective dose combinations anti-Acute myocardial ischemia.Objective:to Explore best dosage of Yang Xin Tong Mai effective component effective Recipe.Methods:Randomly divided 72 Wistar rats into 9 groups,According L4(3⁴).orthogonal design and arrange the experiments.Every factor take 3 different level.All groups were established with Pit inject via caudal vein.Every rat was examined15s,30s,2min,5min,10min electrocardiogram ECG after they were in narcosis,then take Carotid artery blood to examine Whole blood viscosity and Plasma viscosity.Result and Conclusion:select Intuitive statistical analysis method to analysis the result,5 effective component effective dose should like follows,The Ginsenoside:34.33g/kgbw, tanshinoneâ…¡A:1.27g/kgbw,ginseng polysaccharide:4.77g/kgbw,the total alkaloid: 0.67g/kgbw,the total volatile oil:62.2ml/kgbw.Partâ…¢Experimental study of Yang Xin Tong Mai effective component Recipe promote angiogenesis of HSBBExperiment 1:Experimental study the VEGF,bFGF expression of HSBB rats treated by Yang Xin Tong Mai effective component RecipeObjective:to explore the mechanism of Yang Xin Tong Mai effective component Recipe effective component recipe anti-HBSS.Methods:Randomly divided 24 Wistar rats Successful model established by Ligation of the left anterior descending coronary artery into 4 groups:model group,YTâ… therapy group, YTâ…¡therapy group,SXBXW therapy group and add the sham group,health group,all is 6groups.Every group feed Standard feed and gavages corresponding recipe 4ml/qd for 2weeks. To the 15th day,animals were sacrificed and myocardial tissue samples were frozen in liquid nitrogen.Immunohistochemistry used to detect the expression of CD34,VEGF,bFGF in myocardial tissue.Result:â‘ MVC:YTâ… therapy group,YTâ…¡therapy group,SXBXW therapy group compared with the model groups have significant statistical significance(P＜0.05).â‘¡VEGF expression:YTâ… therapy group,YTâ…¡therapy group,SXBXW therapy group compared with the model groups have significant statistical significance(P＜0.05).â‘¢bFGF expression:YTâ… therapy group,YTâ…¡therapy group,SXBXW therapy group compared with the model groups have significant statistical significance(P＜0.05).Conclusion:Promote VEGF,bFGF increase in the synthesis and secretion caused ischemic angiogenesis,thereby establishing a good collateral circulation maybe the important mechanisms of Yang Xin Tong Mai effective component Recipe to treat HBSS.Experiment 2:Experimental study the VEGFmRNA,bFGFmRNA expression of HSBB rats treated by Yang Xin Tong Mai effective component recipeObjective:to explore the mechanism of Yang Xin Tong Mai effective component Recipe effective component recipe anti-HBSS.Methods:Randomly divided 24 Wistar rats Successful model established by Ligation of the left anterior descending coronary artery into 4 groups:model group,YTâ… therapy group, YTâ…¡therapy group,SXBXW therapy group and add the sham group,health group,all is 6groups.Every group feed Standard feed and gavages corresponding recipe 4ml/qd for 2weeks. To the 15th day,animals were sacrificed and myocardial tissue samples were frozen in liquid nitrogen.RT-PCR and agarose gel electrophoresis used to detect the expression of VEGFmRNA,bFGFmRNA.Result:â‘ VEGFmRNA expression:YTâ… therapy group,YTâ…¡therapy group,SXBXW therapy group compared with the model groups have significant statistical significance(P＜0.05).â‘¡bFGFmRNA expression:YTâ… therapy group,YTâ…¡therapy group,SXBXW therapy group compared with the model groups have significant statistical significance(P＜0.05).Conclusion:Promote VEGFmRNA,bFGFmRNA up to VEGF,bFGF increase in the synthesis and secretion caused ischemic angiogenesis,thereby establishing a good collateral circulation maybe the important mechanisms of Yang Xin Tong Mai effective component Recipe to treat HBSS.Experiment 3:Experimental study the impact of Coronary microcirculation and Factors expression of HSBB rats treated by Yang Xin Tong Mai effective component RecipeObjective:to explore the mechanism of Yang Xin Tong Mai effective component Recipe effective component recipe anti-HBSS by research on Coronary microcirculation and related factors expression.Methods:Randomly divided 128 Wistar rats Successful model established by Ligation of the left anterior descending coronary artery into 4 groups:model group,YTâ…¡injection group, Fufang Danshen injection group and add the sham group.After 10mins since modeling inject the corresponding injection by external jugular vein,then ink perfusion,fluorescence Methods were used to observe coronary microcirculation;Carotid artery blood be used to test the MDA, Na⁺-K⁺-ATPase,Ca2⁺-ATPase,TXB2,6-K-PGF1α,NO.Result:â‘ Capillary perfusion of ink:YTâ…¡injection therapy group compared with model group, Fufang Danshen injection therapy group,the sham group has significant statistical significance (P＜0.05 or P＜0.01).â‘¡AT,FT,ST:YTâ…¡injection group,model group,Fufang Danshen injection group,compared with the sham group is more slowly(P＜0.01);The AT of YTâ…¡injection therapy group is shorter than model group;the AT and ST of Fufang Danshen injection group are longer than he sham group's(p＜0.01).â‘¢MDA,Na⁺-K⁺-ATPase,Ca²⁺-ATPase,TXB₂,6-K-PGF1α,NO:YTâ…¡injection therapy group compared with other 3 groups has significant statistical significance(P＜0.05 or P＜0.01).Conclusion:Yang Xin Tong Mai effective component promote angiogenesis maybe have relation with anti-free radicals,reducing calcium overload,Adjustment of endothelial function.