Comparison On Therapeutic Effects By Electro-acupuncturing Different Points On Rats With Acute Cerebral Ischemia And Study Of The Related Mechanism

Objective:By electro-acupuncturing renzhong,Baihui points of Du Meridian, Ganshu,Shenshu points of Beishu points,Quchi,Zusanli points of Yangrning Meridians,we compared their acupuncturing effects on the volume of cerebral infarction,neuron density of the model rats with the middle cerebral artery occlusion(MCAO),by analyzing NO metabolism,ET and neuropeptide CGRP, NPY and NPY gene express of model rats with MCAO,we explored the mechanism of electro-acupuncturing treating the acute cerebral ischemia injury.Methods:75 male SD rats were randomly divided five groups.i.e., pseudo-operation group,model group,Renzhong,Baihui group,Ganshu,Shenshu group and Quchi,Zusanli group.Each group had 15 rats.To establish the MCAO model,we used TTC manipulation experience to measure the cerebral infarction's volume,immunohistochemistry and HE manipulation experience to evaluate neuron density of Hippocampus CA1 area and cerebral cortexⅢ-Ⅳ, nitrate reductase to detect NO,colorinetric assay to inspect NOS, radioimmunoassay to check ET,CGRP and NPY;RT-PCR to examine NPYmRNA express. Results:1.After rats with MCAO had acute cerebral ischemia for 72 hours, compared with pseudo-operation group,we found nerve malfunction progressively accerated,cerebral infarction appeared in the side focus on disease and neuron density of Hippocampus CA1 area and cerebral cortexⅢ-Ⅳdecreased,the contents of NO,NOS,ET and NPY in brood and brain tissue apparently increased, however,the content of CGRP apparently decreased.(p＜0.05 or p＜0.01)2.After rats with MCAO had acute cerebral ischemia for 72 hours,compared all electro-acupuncturing groups with model group,we found:Electroacupuncturing in Renzhong,Baihui group and Ganshu,Shenshu group could improve the behavior score to some extent,moreover,it took effect after ischemia for 48 hours,meanwhile,the volume cerebral infarction reduced and neuron density of Hippocampus CA1 area and density of cerebral cortexⅢ-Ⅳincreased (p＜0.05 or p＜0.01).Besides the results above,we also found that the content of NO in serum and content of NOS in brain tissue decreased while NO in brain tissue and NOS in serum have no any change(P＞0.05).The contents of NPY in plasma and brain tissue and ET in brain tissue decreased while the content of ET in plasma have no apparent change(p＞0.05).Moreover,the content of CGRP in plasma and brain tissue also increased(p＜0.05 or p＜0.01).If we compared the results of both group,it is not significant(P＞0.05).Electro-acupuncturing in Quchi,Zusanli group has no apparent effect on the parameters above(p＞0.05).3.After rats with MCAO had acute cerebral ischemia for 72 hours,compared with pseudo-operation group,we found:the relative density of NPYmRNA in brain tissue apparently increased(P＜0.01).Compared all electro-acupuncturing groups with model group,we also found that electro-acupuncturing in the Renzhong,Baihui group,Ganshu,Shenshu group would apparently decreased the relative density of NPYmRNA in brain tissue(P＜0.05).Compared with the results of both groups,it is no significance(P＞0.05).However,elctro-acupuncturing in Quchi,Zusanli group has no apparent effect on the parameters above(P＞0.05).Conclusions:1.After rats with MCAO had acute cerebral ischemia for 72 hours, the contents of NO,NOS,ET and NPY in brood and brain tissue apparently rise, however,the content of CGRP apparently falls down.It suggests that the abnormal change of the substances above is related to the pathology mechanism of acute cerebral ischemia.2.Electro-acupuncturing in Renzhong,Baihui group and Ganshu,Shenshu group can stabilize and improve the behavior score to some extent(it takes effect after acute cerebral ischemia for 48 hours),reduce the volume cerebral infarction and increase neuron density of Hippocampus CA1 area and cerebral cortexⅢ-Ⅳ. Moreover,Electro-acupuncturing can also bring the protective effect on rats with MCAO having acute cerebral ischemia.However,To restrain NO,NOS,ET,NPY and To promote the synthesis,excretion and secretion of CGRP are one of possible protective mechanisms.And the effect arisen by electro-acupuncturing is the same in both groups. 3.Electro-acupuncturing in the Quchi,Zusanli group has no apparent effects on acute cerebral ischemia of Rats.This should be attributed to treat the Weizheng only acupuncturing the Yangming meridians.It is possible that electro- acupuncturing on the two points above is suitable to treat cerebral ischemia injury recovery and sequela.We need further validate it.4.Inhibiting NPYmRNA express of the focus on MCAO rats' brain tissue is probably one of the molecular mechanisms of the protective effect on the acute cerebral ischemia injury by electro-acupuncturing renzhong and Baihui points of Du Meridian,Ganshu and Shenshu points of Beishu points.