Study On The Behavioral And Blood Plasma NO, NOS, CGRP Content Of Huoxue Xifeng Method Of Migraine Model Rats

Purpose: by observing the blood Huoxuexifeng particles on preventive medication ofmigraine rats after building the behavioural and vascular active substances in the plasma nitricoxide, nitric oxide synthase, the influence of the content of calcitonin gene related peptide,explore the mechanism of Huoxuexifeng prevention and treatment of migraine.Methods:1. The rats were randomly divided into7groups: blank group, model group,west than spirit, town brain group, huoxue extinguish wind particles of low, medium and highdose group.2. Each group preventive lavage for medicine, respectively, in the seventh day dosagegroups after subcutaneous injection of nitroglycerin injection mould, blank group was givenamount of saline solution.3. The building after the completion of the behavior of rats were observed.4. To be put to death all the experimental rats after behavioral observation, usingenzyme-linked immunosorbent assay to detect each group rats, the content of NO, NOS andCGRP in plasma observation invigorate the extinguish wind granule on migraine vascularactive substances in the plasma of rats.5. By statistical calculation prove that invigorate the extinguish wind granule on ratmodel of migraine migraine symptoms have improved effect and of NO, NOS and CGRP inplasma.Results:1. Blood extinguish wind granule on the impact of migraine rats behavior:climbing cage, compared with model group, huoxue extinguish wind particles of high, middledose group and west town brain rather than the group spirit and the group of rats climbingcage number decreased significantly (P <0.05), and no obvious difference between the abovefour groups (P>0.05); Number of months, compared with model group, huoxue extinguishwind particles of high, middle dose group of group of rats with sibeline scratching his headsignificantly reduced (P <0.05), and no obvious difference between the above three groups(P>0.05).2. Blood extinguish wind granule on migraine rats plasma impact: the levels of NO, NOSand CGRP in compared with model group, the treatment group decreased the NO content in plasma of rats (P <0.05); The content of NOS also significantly reduced (P <0.05); Thecontent of CGRP in addition to invigorate the extinguish wind low dose group had a tendencyto reduce, there were no significant differences between the groups (P>0.05).Conclusion:1. The subcutaneous injection of nitroglycerin injection made moldpreparation methods could be successfully migraine animal model in rats.2. Invigorate the extinguish wind particles can affect the behavior of migraine rats modeland NO, NOS and the content of CGRP in plasma.3. The qi and extinguish wind granule is an effective drug for prevention and treatment ofmigraine rats, deserves further research and development.