| Based on the Traditional Chinese medicine compatibility theory,study on documents and clinical practice,to offer pathogenetic Hypothesis is put forward that the stasis of blood circulation and body fluid metabolism influenced each other,leading to lumbar intervertebral discprolapse.To optimize the TCM diminutive complex prescription for lumbar intervertebral discprolapse is suggested.The effect of Huoxue Lishui fang has been proved through Pharmacodynamic studies.To discuss the mechanism of lumbar intervertebral discprolapse intervened by You Hua Fang will provide a theoretical basis and new approach for clinical treatment.Objective:The best combination of various Chinese medicine will be drawn by formulation optimization of Huoxue Lishui Fang,combining the clinical treatment experience and study on documents.Therefore the effect of Huoxue Lishui fang will be tested.The pathogenetic Hypothesis will also be verified that the stasis of blood circulation and body fluid metabolism influence each other,leading to lumbar intervertebral discprolapse.This essay will provide a basis and method for clinical treatment by discussion of the mechanism of lumbar intervertebral discprolapse intervened by You Hua Fang.Method:By way of computer and manual searching documents,the pathogenesis, treatment and prescription of lumbar intervertebral discprolapse have been collected,collated,analyzed and summarized.The TCM diminutive complex presciption Composed by 5 herbs of Chinese medicine will be selested based on collected prescription,clinical treatment experience,the frequency of using Chinese medicine,Chinese medicine inter-compatibility relations,and the pathogenetic that the stasis of blood circulation and body fluid metabolism influence each other.Experimental animals were divided into the control group,the Chinese Medicines Group and the aspirin group NS,Decoction of Chinese medicine and aspirin were given separately.Take the writhing number of the mice induced by acetic acid,swelling degree of mouse ear induced by xylene and swelling degree of rats' limb induced by Chicken egg white as evaluating index to conducte Pharmacodynamic study,thus undertaking the formulation optimization prescri—ption applying the homogeneous design. SD(Sprague-dawley) rats were randomly divided into the control group,model group,the Chinese Medicines Group and the aspirin group.Design the models of lumbar interver—tebral discprolapse,using aspirin as the positive control drug.Discuss the mechanism of lumbar intervertebral discprolapse intervened by You Hua Fang by testing rats' mechanical stimulation pain threshold,testing the MMP-3,TIMP-1 in transplanted nucleus pulposus of transplantation of autologous nucleus pulposus in rats,investigating the change of histology.Result:Obtain the TCM diminutive complex presciption by preliminary screening and draw the best combination and ratio of various Chinese medicine in the prescription.The best formula and ratio are as follows:1:40g;2:30g; 3:20g;4:0g;5:35g;Huoxue Lishui Fang significantly reduced the writhing number of the mice induced by acetic acid,meanwhile alleviate swelling degree of mouse ear induced by xylene;You Hua Fang improved the rats' left hind mechanical stimulation pain threshold significantly,and also relieved pathological damage of nucleus pulposus and nerve root,downregulated the expression of MMP-3 Protein and mRNA and up-regulated the expression of TIMP-1 Portein and mRNA in transplanted nucleus pulposus,improved MMP-3/TIMP-1 ratios disequilibrium.Conclusion:The formula screening and optimization is feasible by applying prescription database,homogeneous design,combining with expertise and specific experiement result.You Hua Fang has significant anti-inflammatory and analgesic effect on Non-compressive model study of experimental lumbar nucleus pulposus prolapse breeding nerve root pain in rats by Transplantation of Autologous Nucleus Pulposus.Its function in relieving lumbar intervertebral disc degeneration and nerve root pain is probably related to improving MMP-3/TIMP-1 disequilibrium.
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