The Effect Of Ambroxol On Psendomonas Aernginosa Biofilm In Vitro And In Vivo

Pseudomonas aeruginosa (P.a) is an important opportunistic human pathogen, causing various infections that are often very persistent. P.a infections are the major cause of death in cystic fibrosis patients. These infections are difficult to treat since P.a is resistant to most antibiotics and its antibiotic susceptibility is decreased when it is present in biofilms. P.a produces many exoproducts (including toxins and hydrolytic enzymes) that are involved in virulence. Recent research has elucidated many mechanisms and pathways that regulate the production of these virulence factors. The regulation is extremely complex and many components are influenced by environmental conditions. Quorum sensing (QS) is a key regulatory system, which itself is affected by many other regulators.Targeting the regulation of pathogenicity factors provides a novel strategy for combating P.a infections.In our experimentation, the BF model of P.a is established in vitro, to explore the relationship between BF formation and QS system; The inhibitory effect of ambroxol on adhesion and the BF formation of P.a on the carrier surface is Observed; The damage effect of ambroxol on the BF of P.a and its impact on BF after combining with levofloxacin (LFX) is evaluated. It is observed that the inhibition of the Ambroxol on P.a virulence factor expression and the anti biofilm infection mechanisms of ambroxol.Through the establishment of intra-abdominal infection in rats in vivo,the role ambroxol acted on P.a biofilm infection and the possible mechanism were explored.