| IntroductionCoronary heart disease is the main serious disease which is harmful to health, of them acute myocardial infarction (AMI) is the serious type. With the development of Intravenous thrombolysis,Percutaneous transluminal coronary angioplasty, ischemic myocardial injury was significantly reduced. But the reperfusion led to ischemia/reperfusion injury(I/RI). Ischemic postconditioning was proved to reduce I/RI through the PI3K/Akt pathway play a heart protective effect.Diabetes has become a serious disease to human health. Atherosclerosis was main chronic complication. However, the cardioprotective effect and mechanism of ischemic postconditioning on atherosclerosis have not been verifed.In the present experiment, we studied the effect on cardiovascular system and expression of Akt,eNOS in the heart of diabetic rats.Materials and methodsSD rats(12-week-old males) were randomly divided into 6 groops of 8 rats each, Diabetic model was induced by Streptozotozin (STZ,45mg/kg):normal; sham; IR IPostC; IR+wortmannin; IPostC+wortmannin. The effects of IPostC on I/RI were evaluated by infarct size, apoptotic index, and gene expression analysis(p-Akt, Akt, p-eNOS, eNOS protein and mRNA expression). Results1,IPostC can reduce infarct size, apoptotic index.2,IPostC can increase p-Akt, Akt, p-eNOS, eNOS protein and mRNA the expression.3,With the wortmannin, the inhibitor of PI3K/Akt pathway, IPostC can not reduce infarct size, apoptotic index and the expression of p-Akt, Akt, p-eNOS, eNOS protein and mRNA were lessened.ConclusionIPostC has the certain protective effects on I/RI of diabetic rats. Wortmannin reduced the protective effects of IPostC. So the effects of IPostC on I/RI of diabetic rats may be mediated by the PI3K/Akt pathway. |
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