Study Of Protective Effect Of Post-conditioning On Rat Hepatic Ischemia-Reperfusion Injury

Objective:To investigate the protective effect of ischemic postconditioning (IPo)on hepatic ischemia-reperfusion injury and the expressions of eNOS in rats.Methods:With a rat model of acute liver ischemia-reperfusion,75 healthy male Sprague-Dawley rats were randomly divided into shamoperated(S), ischemia-reperfusion(IR)and IPo group.Before persistent reperfusion,animals were given six brief reperfusion-ischemia in IPo group.The sample of blood and hepatic tissue of all groups were taken after experiment.Results:Compared with IR group,the concentrations of both aspartate aminotransferase(AST)and alanine aminotransferase(ALT),the concentrations of malondialdehyde(MDA)in hepatic tissue in IPo group decreased markedly(P＜0.01), while the concentrations of superoxide dismutase(SOD)increased significantly(P＜0.01),the hepatic congestion and pathological damage also relieved obviously.The expression of eNOS protein in Ipo group was significantly higher than the two remaining groups(p＜0.01).Conclusion:IPo can attenuate hepatic ischemia-reperfusion injury by activating the prosurvival kinases eNOS in accordance with the RISK pathway and reducing the concentrations of oxygen free radical.