Foundmental Research Of The Sorption Of Flavonoids In Scutellaria Baicalensis Georgi And Microcos Paniculata L. On Macroporous Resins

Fundamental research of the sorption of herbal active ingredient on macroporous resins plays an important role on its industrial application. As flavonids in Scutellaria baicalensis Georgi and Microcos paniculata L. as studied objects, this paper systemly inverstigated some common problems, such as the adsorption characteristic of macroporous resins on flavonoids, the sorption kinetics and thermodynamics, the fixed bed and fluidized bed sorption process, and the computer simulation of adsorption process.Study found that under the setted conditions, flavonids in Scutellaria baicalensis Georgi and Microcos paniculata L. sorption on macroporous resin similar monolayer adsorption, Langmuir equation could well describe their adsorption isotherms,which equivalent adsorption△H located between-75 and-5 KJ.mol-1, the free energy change△G at-5～-2 KJ.mol-1, the entropy change AS between-0.08 and-0.01 KJ.mol"1. K-1; its characteristic separation factor RL between 0 and 1, and the adsorption process of is the physical spontaneous adsorption process. Meanwhile, the Kannan model can describe the sorption kinetics of flavonoids in Scutellaria baicalensis Georgi on AB-8 and HPD-100 resin; and the adsorption process of flavonoids in Microcos paniculata L.on D101, NKA and HPD300 resin fitted the Lagergren kinetic model; their absorption process affected by the film diffusion and particle diffusion, but the control step is the film diffusion process. The study also found that the kinetics of total flavonoids adsorption fitted the general laws of thermodynamics and kinetics, but affected by the interaction between the adsorbates, while, the adsorption kinetics of vitexin and isorhamnetin-3-O-β-rutinoside lack of regularity.In addition, through a comparative study of the fixed bed adsorption process and the fluidization adsorption process, due to the reduction of the film diffusion resistance, the equilibrium adsorption time was significantly shorter and the elution time was significantly lower for the latter, the flavonoids in Scutellaria baicalensis Georgi adsorpted on D101 resin can elution down about 90% in 10 min. For the flavonoids in Microcos paniculata L. when feed flow rate 1.0 BV/h, elution flow rate of 2.0 BV/h, the sample concentration 2.0 mg/mL, eluent pH value of 7.8 and elution solution 2.8 BV, its purity reached 61.77%, yield of 80.25%.In the course of the study, this paper proposed a new method that using the computer software to simulate and predict the sorption process of adsorbents (macroporous resin) and adsorbate (flavonoid molecules). In the computer simulation and experimental study of the non-polar polystyrene skeleton resin and baicalin and baicalein, we found that the sorption process between the resins and compounds can be forecasted through the isosteric heat and average adsorption amouts, and other parameters of the process. The results can set a new way for the research and development of herbal active ingredients purification process by macroporous and provide some new ideas and methods for fundamental research on the population adsorption kinetics and adsorbate interaction of component during the sorption.