The Influence Of Emulsified Isoflurane On Isolated Heart,Saphena Vessel Graft And ICAM-1

ObjectiveTo investigate the cardioprotective effects of emulsified isofluane on ischemia-reperfusion injury in isolated rat heart.Methods32 Sprague-Dawley rats were randomly divided into the following four groups:control group one with STH solution(group B); control group two with 30%Intralipid (group C); high-dose group with emulsified isoflurane 5mmol/1(group A); low-dose group with emulsified isoflurane lmmol/1(group D). Every group were reperfused 60 min at 37℃after these hearts stopped beating and were stopped perfusing for 60min. Left ventricular development pressure(LVDP), maximum rate of pressure increase, minimum rate of pressure decrease (dp/dtmin), coronary flow (CF), myocardial organize was obtained and myocardial ultrastructure were examined under electronic microscope.ResultsThe concentrations of MDA,cTnI in group C and D were much lower than those of control groups (P<0.01). The LVDP were strengthened obviously in group C and D. The concentrations of MDA,cTnI in group A was much highter than other groups (P<0.01). The myocardial ultrastructural changes were significant in these four groups, there were significant edema and severe damage in myocardium in group A;but there was not so severe in group C and group D.ConclusionLow-dose emulsified isoflurane strengthened cardioprotection solution have advantage effect on ischemia-reperfusion injury in isolated rat heart. ObjectiveTo investigate the effect of emulsified isoflurane on the human siaphena vessel graft during the coronary artery bypass grafting.MethodsA fresh saphenous vein obtained from CABG was cut into 2 mm long rings. The rings were randomly divided into four groups. The tension of the vessel rings was studied by the organ chamber technique. These rings were incubated for 1 hour in aerobic Krebs-Herseleit solution(KH); KH with lmmol/1 emulsified isoflurane; KH with intralipid and HTK. The contraction response from 30 nmol/L U46619 and the relaxation response from A23187 were assessed in presence of 7μmol /L indomethacin and 300nmol/L LNNAResultsThe contraction responses from U46619 were almost same among these four groups. Compared with other three groups, the relaxation response from A23187 in group HTK was significant different. Sightly ultrastructral damage was also found in group HTK.Conclusionlmmol/1 emulsified isoflurane would not damage endothelial cells and weak the relaxation response of the saphena vessel significantly. ObjectiveTo investigate the effect of different concentration of emulsified isoflurane on the human siaphena vessel graft during the coronary artery bypass grafting.MethodsA fresh saphenous vein obtained from CABG was cut into 2 mm long rings. The rings were randomly divided into four groups. The tension of the vessel rings was studied by the organ chamber technique. These rings were incubated for 1 hour in aerobic Krebs-Herseleit solution(KH); KH with 1mmol/Lemulsified isoflurane; KH with 2mmol/L emulsified isoflurane and KH with 5mmol/L emulsified isoflurane. The contraction response from 30 nmol/L U46619 and the relaxation response from A23187 were assessed in presence of 7μmol/L indomethacin and 300nmol/L LNNAResultsThe contraction responses from U46619 were almost same among these four groups. Compared with other three groups, the relaxation response from A23187 in group KH with 5mmol/L emulsified isoflurane was significant different. Significant ultrastructral damage was also found in group KH with 5mmol/L emulsified isoflurane.ConclusionHigh concentration emulsified isoflurane would damage endothelial cells and weak the relaxation response of the saphena vessel significantly. ObjectiveTo observe the effect of Emulsified isoflurane on the changes of ICAM-1 expression induced by CPB serum cultured endothelial cells.METHODSICAM-1 expression on cultured endothelial cells was detected by immunohistochemical method.ResultsICAM-1 expression on cultured endothelial cells was increased in every group. ICAM-1 expression in group FBS was the lowest in four groups (P<0.01). ICAM-1 expression in group post-CPB serum and group Emulsified isoflurane was higher than group pre-CPB (P<0.01). ICAM-1 expression in group Emulsified isoflurane was lower than group post-CPB in 1h,2h,4h and 8h. ICAM-1 expression was almost the same between group Emulsified isoflurane and group post-CPB in 12h(P>0.05).ConclusionEmulsified isoflurane could inhibit ICAM-I expression on cultured endothelial cells and might play a role in decreasing systemic inflammatory response syndrome induced by CPB.