The Expression Of The Estrogen Sulfotransferase And Steroid Sulfatase In Endometrial Carcinoma

Background:Endometrial carcinoma is a common malignant tumor of the female reproductive tract, the morbidity of which has been increased recently and the age of which onset is younger than before. Endometrial carcinoma is known as hormone-dependent neoplasm, but, there are many postmenopause women who has low endogenous estrogen in body or no exogenous estrogen supplementary would be suffer endometrial carcinoma. It is considered that in the past estrogen sulfoconjugate is the end products of metabolism,it is thinked to be precursor of biological activity lately, and participate in the modulation of entrogen in vivo. Estrogen sulfotransferase sulfate estrogen to inactive sulfoconjugate, steroid sulfatase hydrolyze the inactive estrogen sulfoconjugate to active estrogen. There were much more reports about EST, STS and breast cancer, while the reports about EST, STS and human endometrial carcinoma were few. This article was going to study the relationship of EST,STS and human endometrial carcinoma.Part one Analysis of clinical manifestation and pathology of endometrial carcinoma between premenopause and postmenopauseObjective:To study the difference of the endometrial carcinoma between premenopause and postmenopause women.Methods:The expression of ER, PR in 109 surgery samples were determined by immunohistochemistry. The relationship between their expressions and menopause, clinical pathology stage, histology differential grade was statistically analyzed.Results:ER, PR expression was 78,86 respectively in 109 cases. Among 36 premenopause cass, there were 29 cases stage one,5 cases stage two,2 cases stage three;12 cases grade one,21 cases grade two,3 cases grade three.25 cases ER positive,32 cases PR positive. Among 73 postmenopause cass, there were 57 cases stage one,7 cases stage two,3 cases stage three,6 cases stage four;16 cases grade one,46 cases grade two,11 cases grade three.53 cases ER positive,54 cases PR positive. No difference statistically were found among clinical manifestation and pathology between premenopause and postmenopause endometrial carcinoma.Conclusion:More than 90% endometrial carcinoma were adenocarcinoma, about 70% were ER positive, more than 70% were PR positive in both groups.Part two The expression of the estrogen sulfotransferase and steroid sulfatase in endometrial carcinomaObjective To study the status of estrogen sulfotransferase (EST),steroid sulfatase(STS) in human endometrial carcinoma.Methods:we examined the expression of estrogen sulfotransferase and steroid sulfatase in 10 normal endometrium and 100 endometrial carcinoma using immunohistochemistry, and the RNA of estrogen sulfotransferase and steroid sulfatase in 9 normal endometrium and 10 endometrial carcinoma using RT-PCR.Results:(1)estrogen sulfotransferase and steroid sulfatase immunoreactivity was detected in 9 of 10(90%),0 of 10(0%) in nomal endometrium;53 of 100(53%),57 of 100(57%) in endometrial carcinoma, respectively. There were significant correlations of the expression of estrogen sulfotransferase, steroid sulfatase between nomal endometrium and endometrial carcinoma(p< 0.05). (2)The RNA expression is:EST 0.24±0.15, STS 0.08±0.06, STS/EST 0.75±1.21 in normal endometrium;EST 0.07±0.06, STS 0.27±0.15, STS/EST 8.08±9.22 in endometrial carcinoma. The RNA expression of EST, STS, STS/EST are significant different between normal endometrium and endometrial carcinoma (p< 0.05).Conclusion:(1)EST, STS and STS/EST were related with human endometrial carcinomas. (2)We examined the RNA of estrogen sulfotransferase and steroid sulfatase in formalin-fixed paraffin-embedded endometrial carcinoma samples using Roche product. Formalin-fixed paraffin-embedded tissues are resourse for molecular study.