The Study Of Histamine 3 Receptor Antagonist On Treatment To Rat Model With Neonatal HIE

Object:the study on H3 acceptor antagonist compound to the neonatal with Hypoxia ischemia brain damage animal model therapeutic action mechanism research. Method:By ligating left side of the carotid artery prepare neonatal Wistar rats HIE model, and treats the animal models with the H3 acceptor antagonist (Thioperamide), and establishes the normal control group, H1, the H2 treatment group, through the comparison brain organization hippocampal area histamine, MDA, SOD and neuron dropsy, apoptosis with necrosis difference, affects the mechanism compared with the brain hippocampal area neuron's protective function of Thioperamide treatment and with H1 and between the H2 acceptor antagonist compound the relevance. Result:selection lives latter on 7th the age newborn Wistar rats, throught left side carotid artery ligation method preparation newborn big rats HIE model, after manufacturing latter 6,24 and 72 hour,we find that between the Normal control group and NS group of H3, H1 and H2 group, in neuron dropsy, the histamine, MDA, SOD and apoptosis and necrosis's comparison, does not have the remarkable difference; Ties up the latter 24 hours in the carotid artery ligation, the neuron damage achieves the peak; In the H1 group, the Dph+Thio group and the H3 acceptor antagonist compound treatment's above test result's comparison does not have the significance difference, does not have the significance difference to the H3 antagonist compound's neuron protective function's influence;In the H2 group, Cim+Thio group and H3 acceptor antagonist compound treatment above target comparison existence significance difference, Indicates the H2 acceptor antagonist compound regarding the H3 antagonist compound neuron protective function existence influence. Conclusion:1st, the histamine H3 acceptor antagonist compound has the protective function for the neonatal with Hypoxia ischemia neuron damage.2nd, the histamine H3 acceptor antagonist compound the protective function which damages to the neonatal rats with Hypoxia ischemia neuron damage is mainly realizes through the histamine H2 acceptor. Regarding the newborn HIE therapeutic schedule which urgent needs to improve has the very important theory guiding sense, provides the further basis for the clinical new medicine research.