Discuss On The Molecule-Mechanism About How Jianpiyishen Medicinal Granule Prevent And Cure The Recrudesces And Metastasis Of Gastric Cancer From The Anoikis' Route Which Induced By Mitochondria

Discuss on the molecule-mechanism about how Jianpiyishen medicinal granule prevent and cure the recrudesces and metastasis of gastric cancer from the Anoikis'route which induced by mitochondriaGastric cancer is one of the common malignant tumors which threaten the people's life and health. Its incidence rate situated on the second place and fatality rate situated on the first place among the malignant tumors in our country. Therefore, it is an important topic to find out medicaments and methods which can cure the Gastric cancer effectively. The TCM therapeutics is an important means of carcinoma's integrative treatment. The Jianpiyishen medicinal granule is developed by my hierophant that based on her clinic experiences and experimental research of many years. It works favorable in practice. This research began with animal experiments; extend to cell experiments and clinic experiments. We adopted the FCM,TUNEL,AV/PI,RT-PCR,IHC......and so on, to probe into the mechanism of how Jianpiyishen medicinal granule prevent and cure the recrudesces and metastasis of gastric cancer after the surgery.The primary disquisition conclusions and harvests are the follows:1. The Jianpiyishen medicinal granule can restrain recrudesce tumors'development, postpone the metastasis in lungs.2. The Jianpiyishen medicinal granule can induce tumors'cell to apoptosis which lies in the metastasis of lungs and recrudesce focuses. It might be the one important effect among the mechanisms which can prevention and cure the tumors'recrudesce and metastasis.3. The Jianpiyishen medicinal granule can promote many proteins' expression, and those proteins link to the approach which induced by mitochondria and can cause cell's apoptosis, such like caspase9,caspase 3,CytC,Smac and Omi/HtrA2. 4. The Jianpiyishen medicinal granule can promote the mRNA of apoptosis-lined proteins such like caspase9,caspase 3,CytC,Smac and Omi/HtrA2's expression. It proved that the TCM also have multi-target effects.5. We established the anoikis cell model of poor differentiation mucinous adenocarcinoma MGC-803 from human and poor differentiation adenocarcinoma BGC-823 from human by six-hole board covered with poly-HEMA. Then we established the MTS cell model of poor differentiation mucinous adenocarcinoma MGC-803 from human and poor differentiation adenocarcinoma BGC-823 from human by improved spinner bottle and agar culture method. The former simulate tumor cell's Anoikis-condition, and the latter simulate tumor cell's internal-condition.6. The rat blood serum of Jianpiyishen medicinal granule can restrain the growth of MGC-803 and BGC-823 tumor cells. There are distinct differences between the rat blood serum of Jianpiyishen medicinal granule and the normal rat blood serum.7. The rat blood serum of Jianpiyishen medicinal granule can induce the apoptosis of MGC-803 MTS and BGC-823 MTS's tumor cells, and the rate of apoptosis in direct proportion to the time.8. The rat blood serum of Jianpiyishen medicinal granule can distinctness depresses the level of mitochondria membrane potential, and interrelates with time.9. The rat blood serum of Jianpiyishen medicinal granule can induce the expressions of caspase9 and caspase3 in the cells of MGC-803 MTS and BGC-823 tumor cells MTS. which may induce the tumor cells to apoptosis.10. The Jianpiyishen medicinal granule can enhance the ability of patients'blood serum to restrain the tumor cells'multiplication.The innovation of this article: 1. We brought forward the assumption that protect the Anoikis is an important mechanism to prevention and cure gastric cancer, and proved it. It is an innovation of train of thought.2. We first used the MTS cell model of MGC803 and BGC823 tumor cells to study the the anoikis and compound of the TCM. There are no reports about the methods we used at home and abroad. It is an innovation of method.3. Smac,Omi/HtrA2 and XIAP which we studied are proteins being investigate among the domain of apoptosis induced by mitochondria actively, but are rarely studied in the field of gastric cancer's research. It is an innovation of index.